Please use this identifier to cite or link to this item: https://doi.org/10.1128/mBio.01056-18
Title: New variant of multidrug-resistant Salmonella enterica serovar typhimurium associated with invasive disease in immunocompromised patients in Vietnam
Authors: Mather, A.E
Phuong, T.L.T
Gao, Y 
Clare, S
Mukhopadhyay, S
Goulding, D.A
Do Hoang, N.T
Tuyen, H.T
Lan, N.P.H
Thompson, C.N
Trang, N.H.T
Carrique-Mas, J
Tue, N.T
Campbell, J.I
Rabaa, M.A
Thanh, D.P
Harcourt, K
Hoa, N.T
Trung, N.V
Schultsz, C
Perron, G.G
Coia, J.E
Brown, D.J
Okoro, C
Parkhill, J
Thomson, N.R
Chau, N.V.V
Thwaites, G.E
Maskell, D.J
Dougan, G
Kenney, L.J 
Baker, S
Keywords: ampicillin
apramycin
beta lactam antibiotic
bleomycin
chloramphenicol
florfenicol
hygromycin B
kanamycin
quinolone derivative
rifampicin
spectinomycin
streptomycin
sulfonamide
tetracycline
trimethoprim
aac(3') IVa gene
aac(6') lb cr gene
aadA1a gene
aadA2 gene
antibiotic resistance
aph3 gene
arr3 gene
Article
bacterial flagellum
bacterial gene
bacterial genome
bacterial transmission
bacterium isolate
blaOXA 1 gene
blaTEM gene
blmS gene
catB3 gene
cladistics
cmlA2 gene
degradation kinetics
dfrA12 gene
enterocolitis
floR gene
hph gene
Human immunodeficiency virus infection
immunocompromised patient
molecular cloning
multidrug resistance
multilocus sequence typing
nonhuman
oqxAB gene
phenotype
plasmid
priority journal
Salmonella enterica serovar Typhimurium
strAB gene
sul1 gene
sul2 gene
sul3 gene
tetB gene
transposon
Viet Nam
Vietnamese
whole genome sequencing
animal
animal salmonellosis
bacteremia
chicken
classification
complication
disease transmission
drug effect
duck
gastroenteritis
genetic variation
genetics
genotype
heterozygote
human
isolation and purification
microbiology
molecular epidemiology
multidrug resistance
pig
Salmonella enterica serovar Typhimurium
salmonellosis
transmission
zoonosis
Animals
Bacteremia
Carrier State
Chickens
Disease Transmission, Infectious
Drug Resistance, Multiple, Bacterial
Ducks
Gastroenteritis
Genetic Variation
Genotype
HIV Infections
Humans
Immunocompromised Host
Molecular Epidemiology
Salmonella Infections
Salmonella Infections, Animal
Salmonella typhimurium
Swine
Vietnam
Whole Genome Sequencing
Zoonoses
Issue Date: 2018
Citation: Mather, A.E, Phuong, T.L.T, Gao, Y, Clare, S, Mukhopadhyay, S, Goulding, D.A, Do Hoang, N.T, Tuyen, H.T, Lan, N.P.H, Thompson, C.N, Trang, N.H.T, Carrique-Mas, J, Tue, N.T, Campbell, J.I, Rabaa, M.A, Thanh, D.P, Harcourt, K, Hoa, N.T, Trung, N.V, Schultsz, C, Perron, G.G, Coia, J.E, Brown, D.J, Okoro, C, Parkhill, J, Thomson, N.R, Chau, N.V.V, Thwaites, G.E, Maskell, D.J, Dougan, G, Kenney, L.J, Baker, S (2018). New variant of multidrug-resistant Salmonella enterica serovar typhimurium associated with invasive disease in immunocompromised patients in Vietnam. mBio 9 (5) : e01056-18. ScholarBank@NUS Repository. https://doi.org/10.1128/mBio.01056-18
Abstract: Nontyphoidal Salmonella (NTS), particularly Salmonella enterica serovar Typhimurium, is among the leading etiologic agents of bacterial enterocolitis globally and a well-characterized cause of invasive disease (iNTS) in sub-Saharan Africa. In contrast, S. Typhimurium is poorly defined in Southeast Asia, a known hot spot for zoonotic disease with a recently described burden of iNTS disease. Here, we aimed to add insight into the epidemiology and potential impact of zoonotic transfer and antimicrobial resistance (AMR) in S. Typhimurium associated with iNTS and enterocolitis in Vietnam. We performed whole-genome sequencing and phylogenetic reconstruction on 85 human (enterocolitis, carriage, and iNTS) and 113 animal S. Typhimurium isolates isolated in Vietnam. We found limited evidence for the zoonotic transmission of S. Typhimurium. However, we describe a chain of events where a pandemic monophasic variant of S. Typhimurium (serovar I:4,[5],12:i:-sequence type 34 [ST34]) has been introduced into Vietnam, reacquired a phase 2 flagellum, and acquired an IncHI2 multidrug-resistant plasmid. Notably, these novel biphasic ST34 S. Typhimurium variants were significantly associated with iNTS in Vietnamese HIV-infected patients. Our study represents the first characterization of novel iNTS organisms isolated outside sub-Saharan Africa and outlines a new pathway for the emergence of alternative Salmonella variants into susceptible human populations. IMPORTANCE Salmonella Typhimurium is a major diarrheal pathogen and associated with invasive nontyphoid Salmonella (iNTS) disease in vulnerable populations. We present the first characterization of iNTS organisms in Southeast Asia and de-scribe a different evolutionary trajectory from that of organisms causing iNTS in sub-Saharan Africa. In Vietnam, the globally distributed monophasic variant of Salmonella Typhimurium, the serovar I:4,[5],12:i:-ST34 clone, has reacquired a phase 2 flagellum and gained a multidrug-resistant plasmid to become associated with iNTS disease in HIV-infected patients. We document distinct communities of S. Typhimurium and I:4,[5],12:i:-in animals and humans in Vietnam, despite the greater mixing of these host populations here. These data highlight the importance of whole-genome sequencing surveillance in a One Health context in understanding the evolution and spread of resistant bacterial infections. © 2018 Mather et al.
Source Title: mBio
URI: https://scholarbank.nus.edu.sg/handle/10635/176036
ISSN: 2161-2129
DOI: 10.1128/mBio.01056-18
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