Please use this identifier to cite or link to this item: https://doi.org/10.3390/s16091397
Title: A simple bodipy-based viscosity probe for imaging of cellular viscosity in live cells
Authors: Su, D
Teoh, C.L
Gao, N 
Xu, Q.-H 
Chang, Y.-T 
Keywords: Cells
Cytology
Dies
Fluorescence
Mitochondria
Probes
Sensors
BODIPY
Cell imaging
FLIM
Fluorescence intensities
Fluorescence lifetime imaging microscopy
Fluorescence lifetimes
Fluorescent probes
Intracellular viscosity
Viscosity
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
boron derivative
solvent
cell survival
chemistry
fluorescence imaging
fluorescence microscopy
HeLa cell line
human
molecular probe
procedures
spectrofluorometry
viscosity
Boron Compounds
Cell Survival
HeLa Cells
Humans
Microscopy, Fluorescence
Molecular Probes
Optical Imaging
Solvents
Spectrometry, Fluorescence
Viscosity
Issue Date: 2016
Publisher: MDPI AG
Citation: Su, D, Teoh, C.L, Gao, N, Xu, Q.-H, Chang, Y.-T (2016). A simple bodipy-based viscosity probe for imaging of cellular viscosity in live cells. Sensors (Switzerland) 16 (9) : 1397. ScholarBank@NUS Repository. https://doi.org/10.3390/s16091397
Abstract: Intracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. The BTV exhibited a significant fluorescence intensity enhancement of more than 100-fold as the solvent viscosity increased. Also, the probe showed a direct linear relationship between the fluorescence lifetime and the media viscosity, which makes it possible to trace the change of the medium viscosity. Furthermore, it was demonstrated that BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM). © 2016 by the authors; licensee MDPI, Basel, Switzerland.
Source Title: Sensors (Switzerland)
URI: https://scholarbank.nus.edu.sg/handle/10635/175249
ISSN: 1424-8220
DOI: 10.3390/s16091397
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