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https://doi.org/10.1128/AAC.02342-16
| Title: | Missense mutations in the unfoldase ClpC1 of the caseinolytic protease complex are associated with pyrazinamide resistance in Mycobacterium tuberculosis | Authors: | Yee, M Gopal, P Dick, T |
Keywords: | clpc1 protein genomic DNA isoniazid messenger RNA proteasome protein pyrazinamide pyrazinoic acid rifampicin unclassified drug pyrazinamide tuberculostatic agent amino acid substitution amino terminal sequence Article bacterium culture broth dilution cell suspension colony formation cryopreservation DNA extraction hydrolysis loss of function mutation missense mutation Mycobacterium tuberculosis nonhuman polymerase chain reaction priority journal single nucleotide polymorphism spontaneous mutation whole genome sequencing analogs and derivatives antibiotic resistance drug effects genetics microbial sensitivity test missense mutation mutation Mycobacterium tuberculosis Antitubercular Agents Drug Resistance, Bacterial Microbial Sensitivity Tests Mutation Mutation, Missense Mycobacterium tuberculosis Pyrazinamide |
Issue Date: | 2017 | Publisher: | American Society for Microbiology | Citation: | Yee, M, Gopal, P, Dick, T (2017). Missense mutations in the unfoldase ClpC1 of the caseinolytic protease complex are associated with pyrazinamide resistance in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy 61 (2) : e02342. ScholarBank@NUS Repository. https://doi.org/10.1128/AAC.02342-16 | Abstract: | Previously, we showed that mutations in Mycobacterium tuberculosis panD, involved in coenzyme A biosynthesis, cause resistance against pyrazinoic acid, the bioactive component of the prodrug pyrazinamide. To identify additional resistance mechanisms, we isolated mutants resistant against pyrazinoic acid and subjected panD wild-type strains to whole-genome sequencing. Eight of the nine resistant strains harbored missense mutations in the unfoldase ClpC1 associated with the caseinolytic protease complex. © 2017 Yee et al. | Source Title: | Antimicrobial Agents and Chemotherapy | URI: | https://scholarbank.nus.edu.sg/handle/10635/175236 | ISSN: | 0066-4804 | DOI: | 10.1128/AAC.02342-16 |
| Appears in Collections: | Staff Publications Elements |
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