Please use this identifier to cite or link to this item: https://doi.org/10.1128/AAC.02342-16
Title: Missense mutations in the unfoldase ClpC1 of the caseinolytic protease complex are associated with pyrazinamide resistance in Mycobacterium tuberculosis
Authors: Yee, M 
Gopal, P 
Dick, T 
Keywords: clpc1 protein
genomic DNA
isoniazid
messenger RNA
proteasome
protein
pyrazinamide
pyrazinoic acid
rifampicin
unclassified drug
pyrazinamide
tuberculostatic agent
amino acid substitution
amino terminal sequence
Article
bacterium culture
broth dilution
cell suspension
colony formation
cryopreservation
DNA extraction
hydrolysis
loss of function mutation
missense mutation
Mycobacterium tuberculosis
nonhuman
polymerase chain reaction
priority journal
single nucleotide polymorphism
spontaneous mutation
whole genome sequencing
analogs and derivatives
antibiotic resistance
drug effects
genetics
microbial sensitivity test
missense mutation
mutation
Mycobacterium tuberculosis
Antitubercular Agents
Drug Resistance, Bacterial
Microbial Sensitivity Tests
Mutation
Mutation, Missense
Mycobacterium tuberculosis
Pyrazinamide
Issue Date: 2017
Publisher: American Society for Microbiology
Citation: Yee, M, Gopal, P, Dick, T (2017). Missense mutations in the unfoldase ClpC1 of the caseinolytic protease complex are associated with pyrazinamide resistance in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy 61 (2) : e02342. ScholarBank@NUS Repository. https://doi.org/10.1128/AAC.02342-16
Abstract: Previously, we showed that mutations in Mycobacterium tuberculosis panD, involved in coenzyme A biosynthesis, cause resistance against pyrazinoic acid, the bioactive component of the prodrug pyrazinamide. To identify additional resistance mechanisms, we isolated mutants resistant against pyrazinoic acid and subjected panD wild-type strains to whole-genome sequencing. Eight of the nine resistant strains harbored missense mutations in the unfoldase ClpC1 associated with the caseinolytic protease complex. © 2017 Yee et al.
Source Title: Antimicrobial Agents and Chemotherapy
URI: https://scholarbank.nus.edu.sg/handle/10635/175236
ISSN: 0066-4804
DOI: 10.1128/AAC.02342-16
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