Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep45230
Title: Structural Basis for pH-mediated Regulation of F-actin Severing by Gelsolin Domain 1
Authors: Fan J.-S. 
Goh H. 
DIng K.
Xue B. 
Robinson R.C. 
Yang D. 
Keywords: actin
calcium
gelsolin
protein binding
binding site
chemistry
human
metabolism
molecular docking
pH
Actins
Binding Sites
Calcium
Gelsolin
Humans
Hydrogen-Ion Concentration
Molecular Docking Simulation
Protein Binding
Issue Date: 2017
Citation: Fan J.-S., Goh H., DIng K., Xue B., Robinson R.C., Yang D. (2017). Structural Basis for pH-mediated Regulation of F-actin Severing by Gelsolin Domain 1. Scientific Reports 7 : 45230. ScholarBank@NUS Repository. https://doi.org/10.1038/srep45230
Abstract: Six-domain gelsolin regulates actin structural dynamics through its abilities to sever, cap and uncap F-actin. These activities are modulated by various cellular parameters like Ca2+ and pH. Until now, only the molecular activation mechanism of gelsolin by Ca2+ has been understood relatively well. The fragment comprising the first domain and six residues from the linker region into the second domain has been shown to be similar to the full-length protein in F-actin severing activity in the absence of Ca2+ at pH 5. To understand how this gelsolin fragment is activated for F-actin severing by lowering pH, we solved its NMR structures at both pH 7.3 and 5 in the absence of Ca2+ and measured the pKa values of acidic amino acid residues and histidine residues. The overall structure and dynamics of the fragment are not affected significantly by pH. Nevertheless, local structural changes caused by protonation of His29 and Asp109 result in the activation on lowering the pH, and protonation of His151 directly effects filament binding since it resides in the gelsolin/actin interface. Mutagenesis studies support that His29, Asp109 and His151 play important roles in the pH-dependent severing activity of the gelsolin fragment. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/175217
ISSN: 20452322
DOI: 10.1038/srep45230
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