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Title: Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
Authors: Kriszt, R
Arai, S
Itoh, H
Lee, M.H 
Goralczyk, A.G 
Ang, X.M
Cypess, A.M
White, A.P
Shamsi, F
Xue, R
Lee, J.Y 
Lee, S.-C
Hou, Y
Kitaguchi, T
Sudhaharan, T
Ishiwata, S
Lane, E.B 
Chang, Y.-T 
Tseng, Y.-H
Suzuki, M
Raghunath, M 
Keywords: fluorescent dye
brown adipocyte
brown adipose tissue
cell culture
endoplasmic reticulum
single cell analysis
Adipocytes, Brown
Adipose Tissue, Brown
Cells, Cultured
Endoplasmic Reticulum
Fluorescent Dyes
Single-Cell Analysis
Issue Date: 2017
Citation: Kriszt, R, Arai, S, Itoh, H, Lee, M.H, Goralczyk, A.G, Ang, X.M, Cypess, A.M, White, A.P, Shamsi, F, Xue, R, Lee, J.Y, Lee, S.-C, Hou, Y, Kitaguchi, T, Sudhaharan, T, Ishiwata, S, Lane, E.B, Chang, Y.-T, Tseng, Y.-H, Suzuki, M, Raghunath, M (2017). Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes. Scientific Reports 7 (1) : 1383. ScholarBank@NUS Repository.
Abstract: The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-Type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes. ERthermAC accumulated in the endoplasmic reticulum of BAs and displayed a marked change in fluorescence intensity in response to adrenergic stimulation of cells, which corresponded to temperature change. ERthermAC fluorescence intensity profiles were congruent with mitochondrial depolarisation events visualised by the JC-1 probe. Moreover, the averaged fluorescence intensity changes across a population of cells correlated well with dynamic changes such as thermal power, oxygen consumption, and extracellular acidification rates. These findings suggest ERthermAC as a promising new tool for studying thermogenic function in brown adipocytes of both murine and human origins. © 2017 The Author(s).
Source Title: Scientific Reports
ISSN: 20452322
DOI: 10.1038/s41598-017-00291-9
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