Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-09104-5
Title: Oncofetal HMGA2 effectively curbs unconstrained (+) and (-) DNA supercoiling
Authors: Zhao X. 
Peter S.
Dröge P.
Yan J. 
Keywords: DNA
high mobility group A2 protein
protein binding
recombinant protein
single stranded DNA
chemistry
conformation
DNA replication
DNA supercoiling
fetus
genetics
human
magnetism
metabolism
molecular model
neoplasm
optical tweezers
procedures
DNA
DNA Replication
DNA, Single-Stranded
DNA, Superhelical
Fetus
HMGA2 Protein
Humans
Magnetics
Models, Molecular
Neoplasms
Nucleic Acid Conformation
Optical Tweezers
Protein Binding
Recombinant Proteins
Issue Date: 2017
Citation: Zhao X., Peter S., Dröge P., Yan J. (2017). Oncofetal HMGA2 effectively curbs unconstrained (+) and (-) DNA supercoiling. Scientific Reports 7 (1) : 8440. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-09104-5
Abstract: HMGA2 belongs to the family of the high mobility group (HMG) proteins. It binds DNA via three AT-hook domains to the minor groove of adenine-thymine (AT) rich DNA. Recently, a new function of HMGA2 as a replication fork chaperone that protects stem and cancer cells from replication fork collapse induced by chemotherapeutic agents was uncovered, suggesting a previously uncharacterized binding at replication forks. In this study, we examined HMGA2 binding to four DNA structures relevant to replication forks, namely ds DNA, ss DNA, forked DNA and supercoiled DNA plectonemes. We detected HMGA2 binding to supercoiled DNA at the lowest concentration and this binding mode transiently stabilizes the supercoiled plectonemes against relaxation by type I topoisomerase. Together, these findings suggest a plausible mechanism how fork regression and collapse are attenuated by HMGA2 during replication stress, i.e. through transient stabilization of positively supercoiled plectonemes in the parental duplex. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/175179
ISSN: 20452322
DOI: 10.1038/s41598-017-09104-5
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