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Title: Multimodal assessments of Zika virus immune pathophysiological responses in marmosets
Authors: Lum, F.-M
Zhang, W
Lim, K.-C
Malleret, B 
Teo, T.-H
Koh, J.-J
Lee, K.J
Chua, T.-K
Kam, Y.-W
Yee, W.-X
Huen, I
Tan, J.J.L
Amrun, S.N
Prakash KN, B
Cozzone, P.J
Renia, L 
Lee, P.T.H
Ng, L.F.P 
Keywords: neutralizing antibody
virus antibody
virus RNA
B lymphocyte
CD8+ T lymphocyte
cell line
HEK293 cell line
natural killer cell
Zika fever
Zika virus
Antibodies, Neutralizing
Antibodies, Viral
CD8-Positive T-Lymphocytes
Cell Line
HEK293 Cells
Killer Cells, Natural
RNA, Viral
Zika Virus
Zika Virus Infection
Issue Date: 2018
Citation: Lum, F.-M, Zhang, W, Lim, K.-C, Malleret, B, Teo, T.-H, Koh, J.-J, Lee, K.J, Chua, T.-K, Kam, Y.-W, Yee, W.-X, Huen, I, Tan, J.J.L, Amrun, S.N, Prakash KN, B, Cozzone, P.J, Renia, L, Lee, P.T.H, Ng, L.F.P (2018). Multimodal assessments of Zika virus immune pathophysiological responses in marmosets. Scientific Reports 8 (1) : 17125. ScholarBank@NUS Repository.
Abstract: Animal models that recapitulate the human pathophysiology have been developed as useful research tools. Although laboratory mice are widely used, they are phylogenetically “distant” to humans. New world monkeys, such as the common marmoset (Callithrix jacchus) have steadily gained prominence. In this report, marmosets are explored as an alternate in vivo model to investigate infection and immunity of Zika virus (ZIKV). Multimodal platforms, including ultrasound and magnetic resonance imaging (MRI), flow cytometry, and multiplex microbead immunoassays were established to comprehensively decipher immune responses and pathophysiological outcomes. While ZIKV-infected marmosets had detectable ZIKV RNA load in various body fluids, animals did not develop any observable lesions in their testes and brains as shown by ultrasound and MRI. Immune-phenotyping detected differences in the numbers of B cells, CD8+ T cells and HLADR+ NK cells during the first two weeks of infection. Neutralizing ZIKV-specific antibodies were elicited to high levels and targeted epitopes in the E protein. This study presents a one-stop-shop platform to study infection and pathophysiology in marmosets. While marmoset-specific research tools are being refined, the research values of these animals present them as a good model for immune-based therapies. © 2018, The Author(s).
Source Title: Scientific Reports
ISSN: 20452322
DOI: 10.1038/s41598-018-35481-6
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