Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep20415
Title: Cell contractility arising from topography and shear flow determines human mesenchymal stem cell fate
Authors: Sonam, S 
Sathe, S.R 
Yim, E.K.F 
Sheetz, M.P 
Lim, C.T 
Keywords: 6-(4-(3-(methylsulfonyl)benzylamino)-5-(trifluoromethyl)pyrimidin-2-ylamino)-3,4-dihydroquinolin-2(1H)-one
myosin adenosine triphosphatase
quinolone derivative
sulfone
biophysics
bone development
cell adhesion
cell culture
cell differentiation
cytology
drug effects
extracellular matrix
focal adhesion
human
mechanical stress
mesenchymal stroma cell
metabolism
physiology
Actomyosin
Biophysical Phenomena
Cell Adhesion
Cell Differentiation
Cells, Cultured
Extracellular Matrix
Focal Adhesions
Humans
Mesenchymal Stromal Cells
Osteogenesis
Quinolones
Stress, Mechanical
Sulfones
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: Sonam, S, Sathe, S.R, Yim, E.K.F, Sheetz, M.P, Lim, C.T (2016). Cell contractility arising from topography and shear flow determines human mesenchymal stem cell fate. Scientific Reports 6 : 20415. ScholarBank@NUS Repository. https://doi.org/10.1038/srep20415
Abstract: Extracellular matrix (ECM) of the human Mesenchymal Stem Cells (MSCs) influences intracellular tension and is known to regulate stem cell fate. However, little is known about the physiological conditions in the bone marrow, where external forces such as fluid shear stress, apart from the physical characteristics of the ECM, influence stem cell response. Here, we hypothesize that substrate topography and fluid shear stress alter the cellular contractile forces, influence the genetic expression of the stem cells and hence alter their lineage. When fluid shear stress was applied, human MSCs with higher contractility (seeded on 1 Î 1/4m wells) underwent osteogenesis, whereas those with lower contractility (seeded on 2 Î 1/4m gratings) remained multipotent. Compared to human MSCs seeded on gratings, those seeded on wells exhibited altered alignment and an increase in the area and number of focal adhesions. When actomyosin contractility was inhibited, human MSCs did not exhibit differentiation, regardless of the topographical feature they were being cultured on. We conclude that the stresses generated by the applied fluid flow impinge on cell contractility to drive the stem cell differentiation via the contractility of the stem cells.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/174993
ISSN: 20452322
DOI: 10.1038/srep20415
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