Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep20415
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dc.titleCell contractility arising from topography and shear flow determines human mesenchymal stem cell fate
dc.contributor.authorSonam, S
dc.contributor.authorSathe, S.R
dc.contributor.authorYim, E.K.F
dc.contributor.authorSheetz, M.P
dc.contributor.authorLim, C.T
dc.date.accessioned2020-09-09T02:05:38Z
dc.date.available2020-09-09T02:05:38Z
dc.date.issued2016
dc.identifier.citationSonam, S, Sathe, S.R, Yim, E.K.F, Sheetz, M.P, Lim, C.T (2016). Cell contractility arising from topography and shear flow determines human mesenchymal stem cell fate. Scientific Reports 6 : 20415. ScholarBank@NUS Repository. https://doi.org/10.1038/srep20415
dc.identifier.issn20452322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/174993
dc.description.abstractExtracellular matrix (ECM) of the human Mesenchymal Stem Cells (MSCs) influences intracellular tension and is known to regulate stem cell fate. However, little is known about the physiological conditions in the bone marrow, where external forces such as fluid shear stress, apart from the physical characteristics of the ECM, influence stem cell response. Here, we hypothesize that substrate topography and fluid shear stress alter the cellular contractile forces, influence the genetic expression of the stem cells and hence alter their lineage. When fluid shear stress was applied, human MSCs with higher contractility (seeded on 1 Î 1/4m wells) underwent osteogenesis, whereas those with lower contractility (seeded on 2 Î 1/4m gratings) remained multipotent. Compared to human MSCs seeded on gratings, those seeded on wells exhibited altered alignment and an increase in the area and number of focal adhesions. When actomyosin contractility was inhibited, human MSCs did not exhibit differentiation, regardless of the topographical feature they were being cultured on. We conclude that the stresses generated by the applied fluid flow impinge on cell contractility to drive the stem cell differentiation via the contractility of the stem cells.
dc.publisherNature Publishing Group
dc.sourceUnpaywall 20200831
dc.subject6-(4-(3-(methylsulfonyl)benzylamino)-5-(trifluoromethyl)pyrimidin-2-ylamino)-3,4-dihydroquinolin-2(1H)-one
dc.subjectmyosin adenosine triphosphatase
dc.subjectquinolone derivative
dc.subjectsulfone
dc.subjectbiophysics
dc.subjectbone development
dc.subjectcell adhesion
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectcytology
dc.subjectdrug effects
dc.subjectextracellular matrix
dc.subjectfocal adhesion
dc.subjecthuman
dc.subjectmechanical stress
dc.subjectmesenchymal stroma cell
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectActomyosin
dc.subjectBiophysical Phenomena
dc.subjectCell Adhesion
dc.subjectCell Differentiation
dc.subjectCells, Cultured
dc.subjectExtracellular Matrix
dc.subjectFocal Adhesions
dc.subjectHumans
dc.subjectMesenchymal Stromal Cells
dc.subjectOsteogenesis
dc.subjectQuinolones
dc.subjectStress, Mechanical
dc.subjectSulfones
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.contributor.departmentBIOLOGY (NU)
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1038/srep20415
dc.description.sourcetitleScientific Reports
dc.description.volume6
dc.description.page20415
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