Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep34171
Title: Coexistence of multiple minor states of fatty acid binding protein and their functional relevance
Authors: Yu, B
Yang, D 
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: Yu, B, Yang, D (2016). Coexistence of multiple minor states of fatty acid binding protein and their functional relevance. Scientific Reports 6 : 34171. ScholarBank@NUS Repository. https://doi.org/10.1038/srep34171
Abstract: Proteins are dynamic over a wide range of timescales, but determining the number of distinct dynamic processes and identifying functionally relevant dynamics are still challenging. Here we present the study on human intestinal fatty acid binding protein (hIFABP) using a novel analysis of 15 N relaxation dispersion (RD) and chemical shift saturation transfer (CEST) experiments. Through combined analysis of the two types of experiments, we found that hIFABP exists in a four-state equilibrium in which three minor states interconvert directly with the major state. According to conversion rates from the major "closed" state to minor states, these minor states are irrelevant to the function of fatty acid transport. Based on chemical shifts of the minor states which could not be determined from RD data alone but were extracted from a combined analysis of RD and CEST data, we found that all the minor states are native-like. This conclusion is further supported by hydrogen-deuterium exchange experiments. Direct conversions between the native state and native-like intermediate states may suggest parallel multitrack unfolding/folding pathways of hIFABP. Moreover, hydrogen-deuterium exchange data indicate the existence of another locally unfolded minor state that is relevant to the fatty acid entry process. © The Author(s) 2016.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/174931
ISSN: 20452322
DOI: 10.1038/srep34171
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