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https://doi.org/10.1038/srep35903
| Title: | Genome-wide methylation analysis identified sexually dimorphic methylated regions in hybrid tilapia | Authors: | Wan, Z.Y Xia, J.H Lin, G Wang, L Lin, V.C.L Yue, G.H |
Keywords: | animal CpG island DNA methylation female gene expression profiling gene regulatory network genetics genome genome-wide association study hybridization male metabolism nucleotide repeat phylogeny sequence analysis sexual characteristics skeletal muscle Tilapia Animals CpG Islands DNA Methylation Female Gene Expression Profiling Gene Regulatory Networks Genome Genome-Wide Association Study Hybridization, Genetic Male Muscle, Skeletal Phylogeny Repetitive Sequences, Nucleic Acid Sequence Analysis, RNA Sex Characteristics Tilapia |
Issue Date: | 2016 | Publisher: | Nature Publishing Group | Citation: | Wan, Z.Y, Xia, J.H, Lin, G, Wang, L, Lin, V.C.L, Yue, G.H (2016). Genome-wide methylation analysis identified sexually dimorphic methylated regions in hybrid tilapia. Scientific Reports 6 : 35903. ScholarBank@NUS Repository. https://doi.org/10.1038/srep35903 | Abstract: | Sexual dimorphism is an interesting biological phenomenon. Previous studies showed that DNA methylation might play a role in sexual dimorphism. However, the overall picture of the genome-wide methylation landscape in sexually dimorphic species remains unclear. We analyzed the DNA methylation landscape and transcriptome in hybrid tilapia (Oreochromis spp.) using whole genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq). We found 4,757 sexually dimorphic differentially methylated regions (DMRs), with significant clusters of DMRs located on chromosomal regions associated with sex determination. CpG methylation in promoter regions was negatively correlated with the gene expression level. MAPK/ERK pathway was upregulated in male tilapia. We also inferred active cis-regulatory regions (ACRs) in skeletal muscle tissues from WGBS datasets, revealing sexually dimorphic cis-regulatory regions. These results suggest that DNA methylation contribute to sex-specific phenotypes and serve as resources for further investigation to analyze the functions of these regions and their contributions towards sexual dimorphisms. © 2016 The Author(s). | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/174925 | ISSN: | 20452322 | DOI: | 10.1038/srep35903 |
| Appears in Collections: | Elements Staff Publications |
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