Please use this identifier to cite or link to this item: https://doi.org/10.1186/1475-2875-8-115
Title: Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood
Authors: Sriprawat K.
Kaewpongsri S.
Suwanarusk R.
Leimanis M.L.
Lek-Uthai U.
Phyo A.P.
Snounou G. 
Russell B. 
Renia L. 
Nosten F.
Keywords: article
blood filter
blood sampling
cost
human
leukocyte
leukocyte count
neutrophil
nonhuman
parasitemia
Plasmodium vivax malaria
thrombocyte
thrombocyte count
animal
comparative study
erythrocyte
evaluation
filtration
instrumentation
isolation and purification
leukapheresis
methodology
parasitology
Plasmodium vivax
thrombocytopheresis
Animals
Erythrocytes
Evaluation Studies as Topic
Filtration
Humans
Leukapheresis
Plasmodium vivax
Plateletpheresis
Issue Date: 2009
Publisher: BioMed Central Ltd.
Citation: Sriprawat K., Kaewpongsri S., Suwanarusk R., Leimanis M.L., Lek-Uthai U., Phyo A.P., Snounou G., Russell B., Renia L., Nosten F. (2009). Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood. Malaria Journal 8 (1) : 115. ScholarBank@NUS Repository. https://doi.org/10.1186/1475-2875-8-115
Abstract: Background. Investigations of Plasmodium vivax are restricted to samples collected from infected persons or primates, because this parasite cannot be maintained in in vitro cultures. Contamination of P. vivax isolates with host leukocytes and platelets is detrimental to a range of ex vivo and molecular investigations. Easy-to-produce CF11 cellulose filters have recently provided us with an inexpensive method for the removal of leukocytes and platelets. This contrasted with previous reports of unacceptably high levels of infected red blood cell (IRBC) retention by CF11. The aims of this study were to compare the ability of CF11 cellulose filters and the commercial filter Plasmodipur at removing leukocyte and platelet, and to investigate the retention of P. vivax IRBCs by CF11 cellulose filtration. Methods and Results. Side-by-side comparison of six leukocyte removal methods using blood samples from five healthy donor showed that CF11 filtration reduced the mean initial leukocyte counts from 9.4 × 10 3 per μl [95%CI 5.213.5] to 0.01 × 10 3[95%CI 0.010.03]. The CF11 was particularly effective at removing neutrophils. CF11 treatment also reduced initial platelet counts from 211.6 × 10 3 per μl[95%CI 107.5315.7] to 0.8 × 10 3per μl [95%CI -0.72.2]. Analysis of 30 P. vivax blood samples before and after CF11 filtration showed only a minor loss in parasitaemia ( ≤ 7.1% of initial counts). Stage specific retention of P. vivax IRBCs was not observed. Conclusion. CF11 filtration is the most cost and time efficient method for the production of leukocyte- and platelet-free P. vivax-infected erythrocytes from field isolates. © 2009 Sriprawat et al.; licensee BioMed Central Ltd.
Source Title: Malaria Journal
URI: https://scholarbank.nus.edu.sg/handle/10635/174462
ISSN: 14752875
DOI: 10.1186/1475-2875-8-115
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