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https://doi.org/10.1038/ncomms15028
| Title: | Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models | Authors: | Hwang, I.Y Koh, E Wong, A March, J.C Bentley, W.E Lee, Y.S Chang, M.W |
Keywords: | alanine racemase probiotic agent probiotic agent bacterium biofilm enzyme activity genetic engineering infectivity nematode pathogen probiotics rodent virulence animal experiment animal model animal tissue Article bacterial strain Caenorhabditis elegans controlled study drug efficacy Escherichia coli Escherichia coli Nissle 1917 female genetic engineering in vivo study infection prevention intestine infection mouse nonhuman Pseudomonas infection treatment outcome animal disease model Escherichia coli gastroenteritis genetic engineering genetics Institute for Cancer Research mouse microbiology pathogenicity procedures Pseudomonas aeruginosa Pseudomonas infection transgenic microorganism virulence Animalia Bacteria (microorganisms) Caenorhabditis elegans Escherichia coli Escherichia coli Nissle 1917 Mus Pseudomonas aeruginosa Animals Caenorhabditis elegans Disease Models, Animal Escherichia coli Female Gastroenteritis Genetic Engineering Mice Mice, Inbred ICR Microorganisms, Genetically-Modified Probiotics Pseudomonas aeruginosa Pseudomonas Infections Virulence |
Issue Date: | 2017 | Publisher: | Nature Publishing Group | Citation: | Hwang, I.Y, Koh, E, Wong, A, March, J.C, Bentley, W.E, Lee, Y.S, Chang, M.W (2017). Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models. Nature Communications 8 : 15028. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms15028 | Abstract: | Bacteria can be genetically engineered to kill specific pathogens or inhibit their virulence. We previously developed a synthetic genetic system that allows a laboratory strain of Escherichia coli to sense and kill Pseudomonas aeruginosa in vitro. Here, we generate a modified version of the system, including a gene encoding an anti-biofilm enzyme, and use the probiotic strain Escherichia coli Nissle 1917 as host. The engineered probiotic shows in vivo prophylactic and therapeutic activity against P. aeruginosa during gut infection in two animal models (Caenorhabditis elegans and mice). These findings support the further development of engineered microorganisms with potential prophylactic and therapeutic activities against gut infections. © 2017 The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/174427 | ISSN: | 2041-1723 | DOI: | 10.1038/ncomms15028 |
| Appears in Collections: | Elements Staff Publications |
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