Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-018-03156-5
Title: Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement
Authors: Shi, Y.Z
Xiong, S
Zhang, Y
Chin, L.K
Chen, Y.-Y
Zhang, J.B
Zhang, T.H 
Ser, W
Larson, A
Hoi, L.S
Wu, J.H
Chen, T.N
Yang, Z.C
Hao, Y.L
Liedberg, B
Yap, P.H
Tsai, D.P
Qiu, C.-W 
Liu, A.Q
Keywords: bacterium antibody
biotin
nanoparticle
polystyrene
streptavidin
nanoparticle
aerobiology
antibody
bacterium
efficiency measurement
nanoparticle
reaction kinetics
antibody screening
antigen binding
Article
bacterial cell
bacterial load
chemical interaction
contact angle
Escherichia coli
hydrodynamics
Kramer theory
mean residence time
nanofluidics
nonhuman
optofluidics lattice
particle size
Shigella flexneri
theory
turnaround time
bacterium
chemistry
kinetics
microfluidic analysis
procedures
Bacteria
Kinetics
Microfluidic Analytical Techniques
Nanoparticles
Issue Date: 2018
Publisher: Nature Publishing Group
Citation: Shi, Y.Z, Xiong, S, Zhang, Y, Chin, L.K, Chen, Y.-Y, Zhang, J.B, Zhang, T.H, Ser, W, Larson, A, Hoi, L.S, Wu, J.H, Chen, T.N, Yang, Z.C, Hao, Y.L, Liedberg, B, Yap, P.H, Tsai, D.P, Qiu, C.-W, Liu, A.Q (2018). Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement. Nature Communications 9 (1) : 815. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-03156-5
Abstract: Particle trapping and binding in optical potential wells provide a versatile platform for various biomedical applications. However, implementation systems to study multi-particle contact interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we demonstrate the precise control of particle interactions and functions such as controlling aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer's theory, to 0.6 s, owing to the mechanical interactions among aggregated particles. The optofluidic lattice also enables single-bacteria-level screening of biological binding agents such as antibodies through particle-enabled bacteria hopping. The binding efficiency of antibodies could be determined directly, selectively, quantitatively and efficiently. This work enriches the fundamental mechanisms of particle kinetics and offers new possibilities for probing and utilising unprecedented biomolecule interactions at single-bacteria level. © 2018 The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/174314
ISSN: 2041-1723
DOI: 10.1038/s41467-018-03156-5
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