Please use this identifier to cite or link to this item: https://doi.org/10.3389/fnbeh.2015.00158
Title: Lentiviral silencing of GSK-3β in adult dentate gyrus impairs contextual fear memory and synaptic plasticity
Authors: Chew B. 
Ryu J.R. 
Ng T. 
Ma D. 
Dasgupta A. 
NEO SIN HUI 
Zhao J.
Zhong Z.
Bichler Z. 
Sajikumar S. 
Goh E.L.K. 
Keywords: glycogen synthase kinase 3beta
lentivirus vector
short hairpin RNA
adult
animal cell
animal experiment
animal tissue
anxiety
Article
contextual fear memory
controlled study
dentate gyrus
embryo
gene silencing
hippocampus
learning
locomotion
long term memory
long term potentiation
male
memory consolidation
mouse
nerve cell plasticity
nervous system development
nonhuman
recall
spatial memory
young adult
Issue Date: 2015
Publisher: Frontiers Media S.A.
Citation: Chew B., Ryu J.R., Ng T., Ma D., Dasgupta A., NEO SIN HUI, Zhao J., Zhong Z., Bichler Z., Sajikumar S., Goh E.L.K. (2015). Lentiviral silencing of GSK-3β in adult dentate gyrus impairs contextual fear memory and synaptic plasticity. Frontiers in Behavioral Neuroscience 9 (JUNE) : 158. ScholarBank@NUS Repository. https://doi.org/10.3389/fnbeh.2015.00158
Abstract: Attempts have been made to use glycogen synthase kinase-3 beta (GSK3β) inhibitors for prophylactic treatment of neurocognitive conditions. However the use of lithium, a nonspecific inhibitor of GSK3β results in mild cognitive impairment in humans. The effects of global GSK3β inhibition or knockout on learning and memory in healthy adult mice are also inconclusive. Our study aims to better understand the role of GSK3β in learning and memory through a more regionally, targeted approach, specifically performing lentiviralmediated knockdown of GSK3β within the dentate gyrus (DG). DG-GSK3β-silenced mice showed impaired contextual fear memory retrieval. However, cue fear memory, spatial memory, locomotor activity and anxiety levels were similar to control. These GSK3β-silenced mice also showed increased induction and maintenance of DG long-term potentiation (DG-LTP) compared to control animals. Thus, this region-specific, targeted knockdown of GSK3β in the DG provides better understanding on the role of GSK3β in learning and memory. © 2015 Chew, Ryu, Ng, Ma, Dasgupta, Neo, Zhao, Zhong, Bichler, Sajikumar and Goh.
Source Title: Frontiers in Behavioral Neuroscience
URI: https://scholarbank.nus.edu.sg/handle/10635/174286
ISSN: 16625153
DOI: 10.3389/fnbeh.2015.00158
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