Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12896-016-0285-6
Title: Large-scale production of foot-and-mouth disease virus (serotype Asia1) VLP vaccine in Escherichia coli and protection potency evaluation in cattle
Authors: Xiao, Y
Chen, H.-Y 
Wang, Y
Yin, B 
Lv, C
Mo, X 
Yan, H
Xuan, Y
Huang, Y
Pang, W
Li, X
Yuan, Y.A 
Tian, K.
Keywords: Antibodies
Escherichia coli
Purification
Vaccines
Viruses
E. coli
Fifty percent protection dose (PD50)
Foot and mouth disease
Serotypes
Virus-like particles
Diseases
capsid protein
foot and mouth disease vaccine
immunological adjuvant
neutralizing antibody
protein VP0
protein VP1
protein VP3
unclassified drug
virus like particle vaccine
recombinant protein
virus like particle vaccine
animal experiment
antibody response
antibody specificity
Article
bovine
chromatography
controlled study
drug potency
enzyme linked immunosorbent assay
Escherichia coli
fermentation
foot and mouth disease
Foot and mouth disease virus
gene expression system
nonhuman
protein assembly
protein purification
serotype
single drug dose
vaccination
animal
batch cell culture
biosynthesis
Cattle Diseases
Foot-and-Mouth Disease
genetics
metabolism
procedures
protein engineering
virology
Animals
Batch Cell Culture Techniques
Cattle
Cattle Diseases
Escherichia coli
Foot-and-Mouth Disease
Protein Engineering
Recombinant Proteins
Vaccines, Virus-Like Particle
Issue Date: 2016
Publisher: BioMed Central Ltd.
Citation: Xiao, Y, Chen, H.-Y, Wang, Y, Yin, B, Lv, C, Mo, X, Yan, H, Xuan, Y, Huang, Y, Pang, W, Li, X, Yuan, Y.A, Tian, K. (2016). Large-scale production of foot-and-mouth disease virus (serotype Asia1) VLP vaccine in Escherichia coli and protection potency evaluation in cattle. BMC Biotechnology 16 (1) : 56. ScholarBank@NUS Repository. https://doi.org/10.1186/s12896-016-0285-6
Abstract: Background: Foot-and-mouth disease (FMD) is an acute, highly contagious disease that infects cloven-hoofed animals. Vaccination is an effective means of preventing and controlling FMD. Compared to conventional inactivated FMDV vaccines, the format of FMDV virus-like particles (VLPs) as a non-replicating particulate vaccine candidate is a promising alternative. Results: In this study, we have developed a co-expression system in E. coli, which drove the expression of FMDV capsid proteins (VP0, VP1, and VP3) in tandem by a single plasmid. The co-expressed FMDV capsid proteins (VP0, VP1, and VP3) were produced in large scale by fermentation at 10 L scale and the chromatographic purified capsid proteins were auto-assembled as VLPs in vitro. Cattle vaccinated with a single dose of the subunit vaccine, comprising in vitro assembled FMDV VLP and adjuvant, developed FMDV-specific antibody response (ELISA antibodies and neutralizing antibodies) with the persistent period of 6 months. Moreover, cattle vaccinated with the subunit vaccine showed the high protection potency with the 50 % bovine protective dose (PD50) reaching 11.75 PD50 per dose. Conclusions: Our data strongly suggest that in vitro assembled recombinant FMDV VLPs produced from E. coli could function as a potent FMDV vaccine candidate against FMDV Asia1 infection. Furthermore, the robust protein expression and purification approaches described here could lead to the development of industrial level large-scale production of E. coli-based VLPs against FMDV infections with different serotypes. © 2016 The Author(s).
Source Title: BMC Biotechnology
URI: https://scholarbank.nus.edu.sg/handle/10635/174251
ISSN: 14726750
DOI: 10.1186/s12896-016-0285-6
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s12896-016-0285-6.pdf1.45 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

25
checked on Oct 23, 2020

Page view(s)

15
checked on Oct 22, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.