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https://doi.org/10.1038/s41467-018-03754-3
| Title: | De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis | Authors: | Ding C. Lim Y.C. Chia S.Y. Walet A.C.E. Xu S. Lo K.A. Zhao Y. Zhu D. Shan Z. Chen Q. Leow M.K.-S. Xu D. Sun L. |
Keywords: | long untranslated RNA long untranslated RNA dprdm16 messenger RNA short hairpin RNA transcriptome unclassified drug DNA binding protein long untranslated RNA PRDM16 protein, human transcription factor transcriptome assay correlation fat genetic marker metabolism muscle obesity protein risk assessment RNA adipogenesis adult animal cell animal experiment animal model animal tissue Article brown adipocyte brown adipose tissue cell differentiation cell maturation cold exposure controlled study exon gene expression level gene knockdown genetic conservation high throughput sequencing human human cell in vitro study in vivo study lipid storage mouse nonhuman open reading frame RNA sequence tissue specificity white adipose tissue adipogenesis animal brown adipose tissue cell culture cold conserved sequence cytology genetic marker genetics growth, development and aging metabolism obesity thermogenesis tissue distribution Adipocytes, Brown Adipogenesis Adipose Tissue, Brown Animals Cell Differentiation Cells, Cultured Cold Temperature Conserved Sequence DNA-Binding Proteins Gene Knockdown Techniques Genetic Markers Humans Mice Obesity RNA, Long Noncoding Thermogenesis Tissue Distribution Transcription Factors Transcriptome |
Issue Date: | 2018 | Publisher: | Nature Publishing Group | Citation: | Ding C., Lim Y.C., Chia S.Y., Walet A.C.E., Xu S., Lo K.A., Zhao Y., Zhu D., Shan Z., Chen Q., Leow M.K.-S., Xu D., Sun L. (2018). De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis. Nature Communications 9 (1) : 1329. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-03754-3 | Abstract: | Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development. © 2018 The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/174232 | ISSN: | 2041-1723 | DOI: | 10.1038/s41467-018-03754-3 |
| Appears in Collections: | Elements Staff Publications |
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