Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-018-03754-3
Title: De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis
Authors: Ding C.
Lim Y.C. 
Chia S.Y. 
Walet A.C.E. 
Xu S.
Lo K.A.
Zhao Y.
Zhu D.
Shan Z.
Chen Q.
Leow M.K.-S. 
Xu D. 
Sun L. 
Keywords: long untranslated RNA
long untranslated RNA dprdm16
messenger RNA
short hairpin RNA
transcriptome
unclassified drug
DNA binding protein
long untranslated RNA
PRDM16 protein, human
transcription factor
transcriptome
assay
correlation
fat
genetic marker
metabolism
muscle
obesity
protein
risk assessment
RNA
adipogenesis
adult
animal cell
animal experiment
animal model
animal tissue
Article
brown adipocyte
brown adipose tissue
cell differentiation
cell maturation
cold exposure
controlled study
exon
gene expression level
gene knockdown
genetic conservation
high throughput sequencing
human
human cell
in vitro study
in vivo study
lipid storage
mouse
nonhuman
open reading frame
RNA sequence
tissue specificity
white adipose tissue
adipogenesis
animal
brown adipose tissue
cell culture
cold
conserved sequence
cytology
genetic marker
genetics
growth, development and aging
metabolism
obesity
thermogenesis
tissue distribution
Adipocytes, Brown
Adipogenesis
Adipose Tissue, Brown
Animals
Cell Differentiation
Cells, Cultured
Cold Temperature
Conserved Sequence
DNA-Binding Proteins
Gene Knockdown Techniques
Genetic Markers
Humans
Mice
Obesity
RNA, Long Noncoding
Thermogenesis
Tissue Distribution
Transcription Factors
Transcriptome
Issue Date: 2018
Publisher: Nature Publishing Group
Citation: Ding C., Lim Y.C., Chia S.Y., Walet A.C.E., Xu S., Lo K.A., Zhao Y., Zhu D., Shan Z., Chen Q., Leow M.K.-S., Xu D., Sun L. (2018). De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis. Nature Communications 9 (1) : 1329. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-03754-3
Abstract: Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development. © 2018 The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/174232
ISSN: 2041-1723
DOI: 10.1038/s41467-018-03754-3
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