Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-00094-y
Title: Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro
Authors: Yuan, Y 
Hong, Y 
Keywords: fish protein
somatomedin B
animal
blastoma
cell culture
cell self-renewal
cell survival
cytology
embryology
embryonic stem cell
flow cytometry
fluorescence microscopy
genetics
MAPK signaling
metabolism
molecular cloning
Oryzias
phosphorylation
zebra fish
Animals
Blastomeres
Cell Self Renewal
Cell Survival
Cells, Cultured
Cloning, Molecular
Embryonic Stem Cells
Fish Proteins
Flow Cytometry
Insulin-Like Growth Factor II
MAP Kinase Signaling System
Microscopy, Fluorescence
Oryzias
Phosphorylation
Zebrafish
Issue Date: 2017
Citation: Yuan, Y, Hong, Y (2017). Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro. Scientific Reports 7 (1) : 78. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-00094-y
Abstract: Insulin-like growth factors (IGFs) regulate diverse processes including energy metabolism, cell proliferation and embryonic development. They activate the IGF signaling pathway via binding to cell surface receptors. Here we report an essential role of IGF2 in maintaining the pluripotency of embryonic stem (ES) cell from medaka (Oryzias latipes). The medaka igf2 gene was cloned for prokaryotically expression of IGF2 ligand and green fluorescent protein-Tagged IGF2 namely IGF2:GFP. With flow cytometry analysis, we demonstrated that the IGF2:GFP can bind to the cultured ES cells from medaka and zebrafish respectively. We also verified that IGF2 is able to activate the phosphorylation of Erk1/2 and Akt, and sustain the viability and pluripotency of medaka ES cells in culture. Furthermore, we characterized the binding of IGF2:GFP to freshly isolated blastomeres by fluorescence microscopy and electron microscopy. Most importantly, we revealed the important role of IGF2 in supporting the derivation of blastomeres in short-Term culture. Therefore, Medaka IGF2 is essential for the self-renewal of cultured ES cells and blastomeres from fish embryos. This finding underscores a conserved role of the IGF signaling pathway in stem cells from fish to mammals. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/173956
ISSN: 20452322
DOI: 10.1038/s41598-017-00094-y
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