Please use this identifier to cite or link to this item: https://doi.org/10.1007/s13238-016-0292-3
Title: Subcellular redistribution and sequential recruitment of macromolecular components during SGIV assembly
Authors: Yuan, Y 
Hong, Y 
Keywords: envelope protein
green fluorescent protein
unclassified drug
VP088
VP88
viral protein
animal cell
apoptosis
aquaculture
Article
cell growth assay
cellular distribution
confocal microscopy
controlled study
ectopic expression
flow cytometry
fluorescence microscopy
inoculation
Iridovirus
macromolecule
marine species
nonhuman
Oryzias
priority journal
reverse transcription polymerase chain reaction
RNA isolation
Singapore
Singapore grouper iridovirus
staining
time lapse imaging
viral gene delivery system
virus assembly
Western blotting
animal
cell line
embryonic stem cell
fish disease
genetics
human
Iridoviridae
metabolism
pathology
physiology
virology
virus assembly
Animals
Cell Line
Embryonic Stem Cells
Fish Diseases
Humans
Iridoviridae
Oryzias
Viral Proteins
Virus Assembly
Issue Date: 2016
Citation: Yuan, Y, Hong, Y (2016). Subcellular redistribution and sequential recruitment of macromolecular components during SGIV assembly. Protein and Cell 7 (9) : 651-661. ScholarBank@NUS Repository. https://doi.org/10.1007/s13238-016-0292-3
Abstract: Virus infection consists of entry, synthesis of macromolecular components, virus assembly and release. Understanding of the mechanisms underlying each event is necessary for the intervention of virus infection in human healthcare and agriculture. Here we report the visualization of Singapore grouper iridovirus (SGIV) assembly in the medaka haploid embryonic stem (ES) cell line HX1. SGIV is a highly infectious DNA virus that causes a massive loss in marine aquaculture. Ectopic expression of VP88GFP, a fusion between green fluorescent protein and the envelope protein VP088, did not compromise the ES cell properties and susceptibility to SGIV infection. Although VP88GFP disperses evenly in the cytoplasm of non-infected cells, it undergoes aggregation and redistribution in SGIV-infected cells. Real-time visualization revealed multiple key stages of VP88GFP redistribution and the dynamics of viral assembly site (VAS). Specifically, VP88GFP entry into and condensation in the VAS occurred within a 6-h duration, a similar duration was observed also for the release of VP88GFP-containing SGIV out of the cell. Taken together, VP088 is an excellent marker for visualizing the SGIV infection process. Our results provide new insight into macromolecular component recruitment and SGIV assembly. © 2016, The Author(s).
Source Title: Protein and Cell
URI: https://scholarbank.nus.edu.sg/handle/10635/173823
ISSN: 1674800X
DOI: 10.1007/s13238-016-0292-3
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