Please use this identifier to cite or link to this item: https://doi.org/10.1007/s13238-016-0292-3
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dc.titleSubcellular redistribution and sequential recruitment of macromolecular components during SGIV assembly
dc.contributor.authorYuan, Y
dc.contributor.authorHong, Y
dc.date.accessioned2020-09-01T01:02:58Z
dc.date.available2020-09-01T01:02:58Z
dc.date.issued2016
dc.identifier.citationYuan, Y, Hong, Y (2016). Subcellular redistribution and sequential recruitment of macromolecular components during SGIV assembly. Protein and Cell 7 (9) : 651-661. ScholarBank@NUS Repository. https://doi.org/10.1007/s13238-016-0292-3
dc.identifier.issn1674800X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/173823
dc.description.abstractVirus infection consists of entry, synthesis of macromolecular components, virus assembly and release. Understanding of the mechanisms underlying each event is necessary for the intervention of virus infection in human healthcare and agriculture. Here we report the visualization of Singapore grouper iridovirus (SGIV) assembly in the medaka haploid embryonic stem (ES) cell line HX1. SGIV is a highly infectious DNA virus that causes a massive loss in marine aquaculture. Ectopic expression of VP88GFP, a fusion between green fluorescent protein and the envelope protein VP088, did not compromise the ES cell properties and susceptibility to SGIV infection. Although VP88GFP disperses evenly in the cytoplasm of non-infected cells, it undergoes aggregation and redistribution in SGIV-infected cells. Real-time visualization revealed multiple key stages of VP88GFP redistribution and the dynamics of viral assembly site (VAS). Specifically, VP88GFP entry into and condensation in the VAS occurred within a 6-h duration, a similar duration was observed also for the release of VP88GFP-containing SGIV out of the cell. Taken together, VP088 is an excellent marker for visualizing the SGIV infection process. Our results provide new insight into macromolecular component recruitment and SGIV assembly. © 2016, The Author(s).
dc.sourceUnpaywall 20200831
dc.subjectenvelope protein
dc.subjectgreen fluorescent protein
dc.subjectunclassified drug
dc.subjectVP088
dc.subjectVP88
dc.subjectviral protein
dc.subjectanimal cell
dc.subjectapoptosis
dc.subjectaquaculture
dc.subjectArticle
dc.subjectcell growth assay
dc.subjectcellular distribution
dc.subjectconfocal microscopy
dc.subjectcontrolled study
dc.subjectectopic expression
dc.subjectflow cytometry
dc.subjectfluorescence microscopy
dc.subjectinoculation
dc.subjectIridovirus
dc.subjectmacromolecule
dc.subjectmarine species
dc.subjectnonhuman
dc.subjectOryzias
dc.subjectpriority journal
dc.subjectreverse transcription polymerase chain reaction
dc.subjectRNA isolation
dc.subjectSingapore
dc.subjectSingapore grouper iridovirus
dc.subjectstaining
dc.subjecttime lapse imaging
dc.subjectviral gene delivery system
dc.subjectvirus assembly
dc.subjectWestern blotting
dc.subjectanimal
dc.subjectcell line
dc.subjectembryonic stem cell
dc.subjectfish disease
dc.subjectgenetics
dc.subjecthuman
dc.subjectIridoviridae
dc.subjectmetabolism
dc.subjectpathology
dc.subjectphysiology
dc.subjectvirology
dc.subjectvirus assembly
dc.subjectAnimals
dc.subjectCell Line
dc.subjectEmbryonic Stem Cells
dc.subjectFish Diseases
dc.subjectHumans
dc.subjectIridoviridae
dc.subjectOryzias
dc.subjectViral Proteins
dc.subjectVirus Assembly
dc.typeArticle
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.contributor.departmentBIOLOGY (NU)
dc.description.doi10.1007/s13238-016-0292-3
dc.description.sourcetitleProtein and Cell
dc.description.volume7
dc.description.issue9
dc.description.page651-661
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