Please use this identifier to cite or link to this item: https://doi.org/10.1111/febs.14139
Title: Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments
Authors: Sezgin, E
Azbazdar, Y
Ng, X.W
Teh, C 
Simons, K
Weidinger, G
Wohland, T 
Eggeling, C
Ozhan, G
Keywords: beta catenin
frizzled protein
membrane lipid
Wnt protein
Wnt3a protein
cytoskeleton protein
frizzled protein
hybrid protein
low density lipoprotein receptor related protein 6
photoprotein
Wnt protein
Wnt3 protein
Wnt3a protein
Wnt3a protein, zebrafish
wnt8a protein, zebrafish
zebrafish protein
animal cell
animal experiment
animal tissue
Article
cell membrane
cell migration
controlled study
embryo
HEK293T cell line
Niemann Pick disease
nonhuman
priority journal
protein localization
protein protein interaction
Wnt signaling pathway
agonists
animal
cell culture
cell membrane
CHO cell line
Cricetulus
genetics
HEK293 cell line
human
membrane microdomain
metabolism
nonmammalian embryo
pathology
reporter gene
transgenic animal
tumor cell line
Wnt signaling
zebra fish
Animals
Animals, Genetically Modified
Cell Line, Tumor
Cell Membrane
Cells, Cultured
CHO Cells
Cricetulus
Cytoskeletal Proteins
Embryo, Nonmammalian
Genes, Reporter
HEK293 Cells
Humans
Low Density Lipoprotein Receptor-Related Protein-6
Luminescent Proteins
Membrane Microdomains
Niemann-Pick Diseases
Receptors, Wnt
Recombinant Fusion Proteins
Wnt Proteins
Wnt Signaling Pathway
Wnt3 Protein
Wnt3A Protein
Zebrafish
Zebrafish Proteins
Issue Date: 2017
Citation: Sezgin, E, Azbazdar, Y, Ng, X.W, Teh, C, Simons, K, Weidinger, G, Wohland, T, Eggeling, C, Ozhan, G (2017). Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments. FEBS Journal 284 (15) : 2513-2526. ScholarBank@NUS Repository. https://doi.org/10.1111/febs.14139
Abstract: While the cytosolic events of Wnt/?-catenin signaling (canonical Wnt signaling) pathway have been widely studied, only little is known about the molecular mechanisms involved in Wnt binding to its receptors at the plasma membrane. Here, we reveal the influence of the immediate plasma membrane environment on the canonical Wnt–receptor interaction. While the receptors are distributed both in ordered and disordered environments, Wnt binding to its receptors selectively occurs in more ordered membrane environments which appear to cointernalize with the Wnt-receptor complex. Moreover, Wnt/?-catenin signaling is significantly reduced when the membrane order is disturbed by specific inhibitors of certain lipids that prefer to localize at the ordered environments. Similarly, a reduction in Wnt signaling activity is observed in Niemann–Pick Type C disease cells where trafficking of ordered membrane lipid components to the plasma membrane is genetically impaired. We thus conclude that ordered plasma membrane environments are essential for binding of canonical Wnts to their receptor complexes and downstream signaling activity. © 2017 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
Source Title: FEBS Journal
URI: https://scholarbank.nus.edu.sg/handle/10635/173779
ISSN: 1742464X
DOI: 10.1111/febs.14139
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