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Title: CD40L expression allows CD8+ T cells to promote their own expansion and differentiation through dendritic cells
Authors: Tay, N.Q 
Lee, D.C.P 
Chua, Y.L 
Prabhu, N 
Gascoigne, N.R.J 
Kemeny, D.M 
Keywords: antibody
CD40 ligand
lentivirus vector
adoptive transfer
animal cell
animal experiment
animal model
bone marrow derived dendritic cell
CD8+ T lymphocyte
cell differentiation
cell expansion
cell proliferation assay
dendritic cell
flow cytometry
fluorescence activated cell sorting
gene overexpression
genetic transduction
Influenza A virus
Listeria monocytogenes
polymerase chain reaction
protein expression
T lymphocyte
Issue Date: 2017
Citation: Tay, N.Q, Lee, D.C.P, Chua, Y.L, Prabhu, N, Gascoigne, N.R.J, Kemeny, D.M (2017). CD40L expression allows CD8+ T cells to promote their own expansion and differentiation through dendritic cells. Frontiers in Immunology 8 (NOV) : 1484. ScholarBank@NUS Repository.
Abstract: CD8+ T cells play an important role in providing protective immunity against a wide range of pathogens, and a number of different factors control their activation. Although CD40L-mediated CD40 licensing of dendritic cells (DCs) by CD4+ T cells is known to be necessary for the generation of a robust CD8+ T cell response, the contribution of CD8+ T cell-expressed CD40L on DC licensing is less clear. We have previously shown that CD8+ T cells are able to induce the production of IL-12 p70 by DCs in a CD40L-dependent manner, providing some evidence that CD8+ T cell-mediated activation of DCs is possible. To better understand the role of CD40L on CD8+ T cell responses, we generated and characterized CD40L-expressing CD8+ T cells both in vitro and in vivo. We found that CD40L was expressed on 30-50% of effector CD8+ T cells when stimulated and that this expression was transient. The expression of CD40L on CD8+ T cells promoted the proliferation and differentiation of both the CD40L-expressing CD8+ T cells and the bystander effector CD8+ T cells. This process occurred via a cell-extrinsic manner and was mediated by DCs. These data demonstrate the existence of a mechanism where CD8+ T cells and DCs cooperate to maximize CD8+ T cell responses. © 2017 Tay, Lee, Chua, Prabhu, Gascoigne and Kemeny.
Source Title: Frontiers in Immunology
ISSN: 16643224
DOI: 10.3389/fimmu.2017.01484
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