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https://doi.org/10.1002/jmv.2101
Title: | Transport and budding at two distinct sites of visible nucleocapsids of West Nile (Sarafend) virus | Authors: | Ng, ML Tan, S Chu, JJH |
Keywords: | Science & Technology Life Sciences & Biomedicine Virology flavivirus structural proteins cryo-immunolabeling FLAVIVIRUS KUNJIN INFECTED CELLS NEW-YORK RNA ENCEPHALITIS REPLICATION SUGGESTS MEMBRANE PROTEIN NS1 |
Issue Date: | 1-Dec-2001 | Publisher: | WILEY | Citation: | Ng, ML, Tan, S, Chu, JJH (2001-12-01). Transport and budding at two distinct sites of visible nucleocapsids of West Nile (Sarafend) virus. JOURNAL OF MEDICAL VIROLOGY 65 (4) : 758-764. ScholarBank@NUS Repository. https://doi.org/10.1002/jmv.2101 | Abstract: | It has been difficult to detect and visualize the physical nucleocapsid particles during the replication process of the flaviviruses. The use of cryo-immunoelectron microscopy has clearly revealed the capsid proteins and nucleocapsid particles of West Nile (Sarafend) virus (a flavivirus) for the first time. Physical nucleocapsid particles accumulated in large numbers from 8 hr postinfection. Double immunolabeling of the envelope and capsid proteins showed a close association of these structural proteins for most of the replication cycle. By 10 hr postinfection, budding of nucelocapsids from the plasma membrane was very obvious. Although maturation at the plasma membrane was the dominant mode, during late infection, intracellular maturation into large vacuoles was also observed. © 2001 Wiley-Liss, Inc. | Source Title: | JOURNAL OF MEDICAL VIROLOGY | URI: | https://scholarbank.nus.edu.sg/handle/10635/173618 | ISSN: | 01466615 10969071 |
DOI: | 10.1002/jmv.2101 |
Appears in Collections: | Staff Publications Elements |
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