Please use this identifier to cite or link to this item: https://doi.org/10.4049/jimmunol.178.5.2699
Title: Immunization of flavivirus West Nile recombinant envelope domain III protein induced specific immune response and protection against West Nile virus infection
Authors: Chu, JHJ 
Chiang, CCS 
Ng, ML 
Keywords: Adjuvants, Immunologic
Animals
Antibodies, Viral
Cell Proliferation
Cells, Cultured
CpG Islands
Cross Reactions
Encephalitis Virus, Japanese
Female
Immunization
Interferon-gamma
Interleukin-2
Mice
Mice, Inbred BALB C
Protein Structure, Tertiary
Recombinant Proteins
Th1 Cells
Viral Envelope Proteins
West Nile Fever
West Nile Virus Vaccines
West Nile virus
Issue Date: 1-Mar-2007
Publisher: The American Association of Immunologists
Citation: Chu, JHJ, Chiang, CCS, Ng, ML (2007-03-01). Immunization of flavivirus West Nile recombinant envelope domain III protein induced specific immune response and protection against West Nile virus infection. Journal of Immunology 178 (5) : 2699-2705. ScholarBank@NUS Repository. https://doi.org/10.4049/jimmunol.178.5.2699
Abstract: The domain III of the West Nile virus (WNV) envelope glycoprotein (E) was shown to serve as virus attachment domain to the cellular receptor, and neutralizing Abs have been mapped to this specific domain. In this study, domain III of the WNV E protein (WNV E DIII) was expressed as a recombinant protein and its potential as a subunit vaccine candidate was evaluated in BALB/C mice. Immunization of WNV E Dili protein with oligodeoxynucleotides (CpG-DNA) adjuvant by i.p. injection was conducted over a period of 3 wk. The immunized mice generated high titer of WNV-neutralizing Abs. Murine Ab against WNV E Dili protein was also capable of neutralizing Japanese encephalitis virus. The IgG isotypes generated were predominantly IgG2a in the marine sera against the recombinant protein. Splenocyte cultures from the mice coadministrated with WNV E Dili protein and CpG secreted large amounts of IFN-γ and IL-2 and showed proliferation of T cells in the presence of WNV E DIII protein. Overall, this study highlighted that recombinant WNV E DIII protein delivered in combination with CpG adjuvant to mice generated a Th1 immune response type against WNV and can serve as a potential vaccine to prevent WNV infection. Copyright © 2007 by The American Association of Immunologists, Inc.
Source Title: Journal of Immunology
URI: https://scholarbank.nus.edu.sg/handle/10635/173459
ISSN: 00221767
15506606
DOI: 10.4049/jimmunol.178.5.2699
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