Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-4939-3618-2_8
Title: Propagation of Chikungunya virus using mosquito cells
Authors: Ang, SK 
Lam, S
Chu, JJH 
Keywords: C6/36
CHIKV
Mosquito cells
Serum-free
Virus propagation
Aedes
Alphavirus Infections
Animals
Cell Culture Techniques
Cell Line
Chikungunya virus
Culture Media
Culture Media, Serum-Free
Virus Replication
Issue Date: 1-Jan-2016
Publisher: Springer New York
Citation: Ang, SK, Lam, S, Chu, JJH (2016-01-01). Propagation of Chikungunya virus using mosquito cells. Methods Mol Biol 1426 : 87-92. ScholarBank@NUS Repository. https://doi.org/10.1007/978-1-4939-3618-2_8
Abstract: © Springer Science+Business Media New York 2016. Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that transmits in between a mosquito host vector to a primate host and then back to the mosquito host vector to complete its life cycle. Hence, CHIKV must be able to replicate in both host cellular systems that are genetically and biochemically distinct. The ability to grow and propagate the virus in high titers in the laboratory is fundamentally crucial in order to understand virus replication in different host cellular systems and many other CHIKV research areas. Here, we describe a method on CHIKV propagation using C6/36, a mosquito cell line derived from Aedes albopictus in both serum-containing and serum-free media.
Source Title: Methods Mol Biol
URI: https://scholarbank.nus.edu.sg/handle/10635/173286
ISSN: 10643745
19406029
DOI: 10.1007/978-1-4939-3618-2_8
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