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Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-4939-3618-2_8
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dc.titlePropagation of Chikungunya virus using mosquito cells
dc.contributor.authorAng, SK
dc.contributor.authorLam, S
dc.contributor.authorChu, JJH
dc.date.accessioned2020-08-21T07:10:37Z
dc.date.available2020-08-21T07:10:37Z
dc.date.issued2016-01-01
dc.identifier.citationAng, SK, Lam, S, Chu, JJH (2016-01-01). Propagation of Chikungunya virus using mosquito cells. Methods Mol Biol 1426 : 87-92. ScholarBank@NUS Repository. https://doi.org/10.1007/978-1-4939-3618-2_8
dc.identifier.issn10643745
dc.identifier.issn19406029
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/173286
dc.description.abstract© Springer Science+Business Media New York 2016. Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that transmits in between a mosquito host vector to a primate host and then back to the mosquito host vector to complete its life cycle. Hence, CHIKV must be able to replicate in both host cellular systems that are genetically and biochemically distinct. The ability to grow and propagate the virus in high titers in the laboratory is fundamentally crucial in order to understand virus replication in different host cellular systems and many other CHIKV research areas. Here, we describe a method on CHIKV propagation using C6/36, a mosquito cell line derived from Aedes albopictus in both serum-containing and serum-free media.
dc.publisherSpringer New York
dc.sourceElements
dc.subjectC6/36
dc.subjectCHIKV
dc.subjectMosquito cells
dc.subjectSerum-free
dc.subjectVirus propagation
dc.subjectAedes
dc.subjectAlphavirus Infections
dc.subjectAnimals
dc.subjectCell Culture Techniques
dc.subjectCell Line
dc.subjectChikungunya virus
dc.subjectCulture Media
dc.subjectCulture Media, Serum-Free
dc.subjectVirus Replication
dc.typeArticle
dc.date.updated2020-06-23T08:21:17Z
dc.contributor.departmentMICROBIOLOGY AND IMMUNOLOGY
dc.description.doi10.1007/978-1-4939-3618-2_8
dc.description.sourcetitleMethods Mol Biol
dc.description.volume1426
dc.description.page87-92
dc.published.statePublished
dc.description.redepositCompleted
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