Please use this identifier to cite or link to this item: https://doi.org/10.1186/s13287-020-1566-5
Title: Pulsed electromagnetic fields potentiate the paracrine function of mesenchymal stem cells for cartilage regeneration
Authors: Parate, Dinesh 
Kadir, Nurul Dinah 
Celik, Cenk
Lee, Eng Hin 
Hui, James HP 
Franco-Obregon, Alfredo 
Yang, Zheng
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell & Tissue Engineering
Cell Biology
Medicine, Research & Experimental
Research & Experimental Medicine
Pulse electromagnetic fields
Mesenchymal stem cells
Cartilage
Paracrine
UMBILICAL-CORD BLOOD
INTERLEUKIN-1 RECEPTOR ANTAGONIST
CHONDROGENIC DIFFERENTIATION
STROMAL CELLS
BONE
REPAIR
OSTEOARTHRITIS
PROLIFERATION
INFLAMMATION
STIMULATION
Issue Date: 3-Feb-2020
Publisher: BMC
Citation: Parate, Dinesh, Kadir, Nurul Dinah, Celik, Cenk, Lee, Eng Hin, Hui, James HP, Franco-Obregon, Alfredo, Yang, Zheng (2020-02-03). Pulsed electromagnetic fields potentiate the paracrine function of mesenchymal stem cells for cartilage regeneration. STEM CELL RESEARCH & THERAPY 11 (1) : 46. ScholarBank@NUS Repository. https://doi.org/10.1186/s13287-020-1566-5
Abstract: BACKGROUND: The mesenchymal stem cell (MSC) secretome, via the combined actions of its plethora of biologically active factors, is capable of orchestrating the regenerative responses of numerous tissues by both eliciting and amplifying biological responses within recipient cells. MSCs are "environmentally responsive" to local micro-environmental cues and biophysical perturbations, influencing their differentiation as well as secretion of bioactive factors. We have previously shown that exposures of MSCs to pulsed electromagnetic fields (PEMFs) enhanced MSC chondrogenesis. Here, we investigate the influence of PEMF exposure over the paracrine activity of MSCs and its significance to cartilage regeneration. METHODS: Conditioned medium (CM) was generated from MSCs subjected to either 3D or 2D culturing platforms, with or without PEMF exposure. The paracrine effects of CM over chondrocytes and MSC chondrogenesis, migration and proliferation, as well as the inflammatory status and induced apoptosis in chondrocytes and MSCs was assessed. RESULTS: We show that benefits of magnetic field stimulation over MSC-derived chondrogenesis can be partly ascribed to its ability to modulate the MSC secretome. MSCs cultured on either 2D or 3D platforms displayed distinct magnetic sensitivities, whereby MSCs grown in 2D or 3D platforms responded most favorably to PEMF exposure at 2 mT and 3 mT amplitudes, respectively. Ten minutes of PEMF exposure was sufficient to substantially augment the chondrogenic potential of MSC-derived CM generated from either platform. Furthermore, PEMF-induced CM was capable of enhancing the migration of chondrocytes and MSCs as well as mitigating cellular inflammation and apoptosis. CONCLUSIONS: The findings reported here demonstrate that PEMF stimulation is capable of modulating the paracrine function of MSCs for the enhancement and re-establishment of cartilage regeneration in states of cellular stress. The PEMF-induced modulation of the MSC-derived paracrine function for directed biological responses in recipient cells or tissues has broad clinical and practical ramifications with high translational value across numerous clinical applications.
Source Title: STEM CELL RESEARCH & THERAPY
URI: https://scholarbank.nus.edu.sg/handle/10635/171315
ISSN: 17576512
DOI: 10.1186/s13287-020-1566-5
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