Please use this identifier to cite or link to this item: https://doi.org/10.1002/anie.201702509
Title: Global Mapping of Protein-Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells
Authors: Wang, Danyang 
Du, Shubo 
Cazenave-Gassiot, Amaury 
Ge, Jingyan 
Lee, Jun-Seok 
Wenk, Markus R 
Yao, Shao Q 
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
click chemistry
phosphatidylcholine
photo-crosslinking
protein-lipid interactions
quantitative proteomics
PHOTO-CROSS-LINKERS
MAMMALIAN-CELLS
DISULFIDE-ISOMERASE
IN-VIVO
LINKING
BIOLOGY
Issue Date: 2017
Publisher: WILEY-V C H VERLAG GMBH
Citation: Wang, Danyang, Du, Shubo, Cazenave-Gassiot, Amaury, Ge, Jingyan, Lee, Jun-Seok, Wenk, Markus R, Yao, Shao Q (2017). Global Mapping of Protein-Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56 (21) : 5829-5833. ScholarBank@NUS Repository. https://doi.org/10.1002/anie.201702509
Abstract: © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim The protein–lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein–lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein–phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.
Source Title: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
URI: https://scholarbank.nus.edu.sg/handle/10635/170832
ISSN: 14337851
15213773
DOI: 10.1002/anie.201702509
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