Please use this identifier to cite or link to this item: https://doi.org/10.3390/biom8040151
Title: Analytical Considerations of Stable Isotope Labelling in Lipidomics
Authors: Triebl, Alexander 
Wenk, Markus R 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
lipidomics
stable isotope labelling
mass spectrometry
flux analysis
METABOLIC FLUX ANALYSIS
MASS-SPECTROMETRY APPLICATION
FATTY-ACIDS
BIOLOGICAL SAMPLES
INTERNAL STANDARD
RAPID MEASUREMENT
OLEIC-ACID
C-13
RESOLUTION
TRACERS
Issue Date: 1-Dec-2018
Publisher: MDPI
Citation: Triebl, Alexander, Wenk, Markus R (2018-12-01). Analytical Considerations of Stable Isotope Labelling in Lipidomics. BIOMOLECULES 8 (4). ScholarBank@NUS Repository. https://doi.org/10.3390/biom8040151
Abstract: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. Over the last two decades, lipids have come to be understood as far more than merely components of cellular membranes and forms of energy storage, and are now also being implicated to play important roles in a variety of diseases, with lipid biomarker research one of the most widespread applications of lipidomic techniques both in research and in clinical settings. Stable isotope labelling has become a staple technique in the analysis of small molecule metabolism and dynamics, as it is the only experimental setup by which biosynthesis, remodelling and degradation of biomolecules can be directly measured. Using state-of-the-art analytical technologies such as chromatography-coupled high resolution tandem mass spectrometry, the stable isotope label can be precisely localized and quantified within the biomolecules. The application of stable isotope labelling to lipidomics is however complicated by the diversity of lipids and the complexity of the necessary data analysis. This article discusses key experimental aspects of stable isotope labelling in the field of mass spectrometry-based lipidomics, summarizes current applications and provides an outlook on future developments and potential.
Source Title: BIOMOLECULES
URI: https://scholarbank.nus.edu.sg/handle/10635/170465
ISSN: 2218273X
DOI: 10.3390/biom8040151
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