Please use this identifier to cite or link to this item: https://doi.org/10.3390/biom8040151
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dc.titleAnalytical Considerations of Stable Isotope Labelling in Lipidomics
dc.contributor.authorTriebl, Alexander
dc.contributor.authorWenk, Markus R
dc.date.accessioned2020-06-18T09:53:56Z
dc.date.available2020-06-18T09:53:56Z
dc.date.issued2018-12-01
dc.identifier.citationTriebl, Alexander, Wenk, Markus R (2018-12-01). Analytical Considerations of Stable Isotope Labelling in Lipidomics. BIOMOLECULES 8 (4). ScholarBank@NUS Repository. https://doi.org/10.3390/biom8040151
dc.identifier.issn2218273X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/170465
dc.description.abstract© 2018 by the authors. Licensee MDPI, Basel, Switzerland. Over the last two decades, lipids have come to be understood as far more than merely components of cellular membranes and forms of energy storage, and are now also being implicated to play important roles in a variety of diseases, with lipid biomarker research one of the most widespread applications of lipidomic techniques both in research and in clinical settings. Stable isotope labelling has become a staple technique in the analysis of small molecule metabolism and dynamics, as it is the only experimental setup by which biosynthesis, remodelling and degradation of biomolecules can be directly measured. Using state-of-the-art analytical technologies such as chromatography-coupled high resolution tandem mass spectrometry, the stable isotope label can be precisely localized and quantified within the biomolecules. The application of stable isotope labelling to lipidomics is however complicated by the diversity of lipids and the complexity of the necessary data analysis. This article discusses key experimental aspects of stable isotope labelling in the field of mass spectrometry-based lipidomics, summarizes current applications and provides an outlook on future developments and potential.
dc.language.isoen
dc.publisherMDPI
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiochemistry & Molecular Biology
dc.subjectlipidomics
dc.subjectstable isotope labelling
dc.subjectmass spectrometry
dc.subjectflux analysis
dc.subjectMETABOLIC FLUX ANALYSIS
dc.subjectMASS-SPECTROMETRY APPLICATION
dc.subjectFATTY-ACIDS
dc.subjectBIOLOGICAL SAMPLES
dc.subjectINTERNAL STANDARD
dc.subjectRAPID MEASUREMENT
dc.subjectOLEIC-ACID
dc.subjectC-13
dc.subjectRESOLUTION
dc.subjectTRACERS
dc.typeReview
dc.date.updated2020-06-17T04:24:48Z
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.description.doi10.3390/biom8040151
dc.description.sourcetitleBIOMOLECULES
dc.description.volume8
dc.description.issue4
dc.published.statePublished
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