Please use this identifier to cite or link to this item: https://doi.org/10.1210/en.2018-01020
Title: Metabolism of C-13-Labeled Fatty Acids in Term Human Placental Explants by Liquid Chromatography-Mass Spectrometry
Authors: Watkins, Oliver C 
Islam, Mohammad Omedul
Selvam, Preben 
Pillai, Reshma Appukuttan 
Cazenave-Gassiot, Amaury 
Bendt, Anne K 
Karnani, Neerja 
Godfrey, Keith M
Lewis, Rohan M
Wenk, Markus R 
Chan, Shiao-Yng 
Keywords: Science & Technology
Life Sciences & Biomedicine
Endocrinology & Metabolism
ARACHIDONIC-ACID
DOCOSAHEXAENOIC ACIDS
CORD BLOOD
BINDING
TRANSPORT
TROPHOBLAST
GLUCOSE
GROWTH
CELLS
PROTEINS
Issue Date: 1-Jun-2019
Publisher: ENDOCRINE SOC
Citation: Watkins, Oliver C, Islam, Mohammad Omedul, Selvam, Preben, Pillai, Reshma Appukuttan, Cazenave-Gassiot, Amaury, Bendt, Anne K, Karnani, Neerja, Godfrey, Keith M, Lewis, Rohan M, Wenk, Markus R, Chan, Shiao-Yng (2019-06-01). Metabolism of C-13-Labeled Fatty Acids in Term Human Placental Explants by Liquid Chromatography-Mass Spectrometry. ENDOCRINOLOGY 160 (6) : 1394-1408. ScholarBank@NUS Repository. https://doi.org/10.1210/en.2018-01020
Abstract: Copyright © 2019 Endocrine Society Placental lipid transport and metabolism are poorly understood despite the importance for fetal development and lifelong health. We aimed to explore fatty acid (FA) processing in human villous placental explants from seven uncomplicated term singleton pregnancies delivered by elective cesarean section. Explants were treated with stable isotope-labeled palmitic acid (13C-PA), oleic acid (13C-OA), or docosahexaenoic acid (13C-DHA) for 3, 24, or 48 hours. Stable isotope-labeled lipids synthesized by placental explants from labeled FA were quantified, alongside endogenous unlabeled placental lipids, by liquid chromatography-mass spectrometry. Labeled phosphatidylcholines (PCs), triacylglycerols (TAGs), and phosphatidylethanolamines were detected in explants, whereas labeled lysophosphatidylcholines were found in both explants and conditioned media. 13C-PA was primarily directed into PC synthesis (74% of 13C-PA-labeled lipids), whereas 13C-OA was directed almost equally into PC and TAG synthesis (45% and 53%, respectively, of 13C-OA-labeled lipids). 13C-DHA was only detectable in TAGs. TAGs demonstrated the highest isotopic enrichment for all 13C-FAs with 13C-OA-TAGs comprising .50% of total OA-TAGs (unlabeled and labeled), consistent with TAGs being a labile and accessible reservoir for FA storage. Variations in lipid incorporation were correlated to maternal glycemia and body mass index, suggesting that this experimental model could be used to investigate the effect of maternal factors on placental lipid metabolism. We conclude that lipid metabolic partitioning of freshly imported FAs into labile and less labile lipid reservoirs in placenta is FA dependent. This process may partly mediate the physiological preferential transplacental transfer of particular FAs to the fetus, but may also be implicated in the fetoplacental pathophysiology of maternal metabolic dysfunction.
Source Title: ENDOCRINOLOGY
URI: https://scholarbank.nus.edu.sg/handle/10635/170348
ISSN: 00137227
19457170
DOI: 10.1210/en.2018-01020
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
16 - Submitted manuscript_Metabolism of C-13-Labeled Fatty Acids in Term Human Placental.pdf1.16 MBAdobe PDF

OPEN

Post-printView/Download

SCOPUSTM   
Citations

3
checked on Jul 31, 2020

Page view(s)

35
checked on Jul 30, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.