Please use this identifier to cite or link to this item: https://doi.org/10.1210/en.2018-01020
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dc.titleMetabolism of C-13-Labeled Fatty Acids in Term Human Placental Explants by Liquid Chromatography-Mass Spectrometry
dc.contributor.authorWatkins, Oliver C
dc.contributor.authorIslam, Mohammad Omedul
dc.contributor.authorSelvam, Preben
dc.contributor.authorPillai, Reshma Appukuttan
dc.contributor.authorCazenave-Gassiot, Amaury
dc.contributor.authorBendt, Anne K
dc.contributor.authorKarnani, Neerja
dc.contributor.authorGodfrey, Keith M
dc.contributor.authorLewis, Rohan M
dc.contributor.authorWenk, Markus R
dc.contributor.authorChan, Shiao-Yng
dc.date.accessioned2020-06-18T03:32:32Z
dc.date.available2020-06-18T03:32:32Z
dc.date.issued2019-06-01
dc.identifier.citationWatkins, Oliver C, Islam, Mohammad Omedul, Selvam, Preben, Pillai, Reshma Appukuttan, Cazenave-Gassiot, Amaury, Bendt, Anne K, Karnani, Neerja, Godfrey, Keith M, Lewis, Rohan M, Wenk, Markus R, Chan, Shiao-Yng (2019-06-01). Metabolism of C-13-Labeled Fatty Acids in Term Human Placental Explants by Liquid Chromatography-Mass Spectrometry. ENDOCRINOLOGY 160 (6) : 1394-1408. ScholarBank@NUS Repository. https://doi.org/10.1210/en.2018-01020
dc.identifier.issn00137227
dc.identifier.issn19457170
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/170348
dc.description.abstractCopyright © 2019 Endocrine Society Placental lipid transport and metabolism are poorly understood despite the importance for fetal development and lifelong health. We aimed to explore fatty acid (FA) processing in human villous placental explants from seven uncomplicated term singleton pregnancies delivered by elective cesarean section. Explants were treated with stable isotope-labeled palmitic acid (13C-PA), oleic acid (13C-OA), or docosahexaenoic acid (13C-DHA) for 3, 24, or 48 hours. Stable isotope-labeled lipids synthesized by placental explants from labeled FA were quantified, alongside endogenous unlabeled placental lipids, by liquid chromatography-mass spectrometry. Labeled phosphatidylcholines (PCs), triacylglycerols (TAGs), and phosphatidylethanolamines were detected in explants, whereas labeled lysophosphatidylcholines were found in both explants and conditioned media. 13C-PA was primarily directed into PC synthesis (74% of 13C-PA-labeled lipids), whereas 13C-OA was directed almost equally into PC and TAG synthesis (45% and 53%, respectively, of 13C-OA-labeled lipids). 13C-DHA was only detectable in TAGs. TAGs demonstrated the highest isotopic enrichment for all 13C-FAs with 13C-OA-TAGs comprising .50% of total OA-TAGs (unlabeled and labeled), consistent with TAGs being a labile and accessible reservoir for FA storage. Variations in lipid incorporation were correlated to maternal glycemia and body mass index, suggesting that this experimental model could be used to investigate the effect of maternal factors on placental lipid metabolism. We conclude that lipid metabolic partitioning of freshly imported FAs into labile and less labile lipid reservoirs in placenta is FA dependent. This process may partly mediate the physiological preferential transplacental transfer of particular FAs to the fetus, but may also be implicated in the fetoplacental pathophysiology of maternal metabolic dysfunction.
dc.language.isoen
dc.publisherENDOCRINE SOC
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectEndocrinology & Metabolism
dc.subjectARACHIDONIC-ACID
dc.subjectDOCOSAHEXAENOIC ACIDS
dc.subjectCORD BLOOD
dc.subjectBINDING
dc.subjectTRANSPORT
dc.subjectTROPHOBLAST
dc.subjectGLUCOSE
dc.subjectGROWTH
dc.subjectCELLS
dc.subjectPROTEINS
dc.typeArticle
dc.date.updated2020-06-17T04:16:22Z
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.contributor.departmentDEPT OF OBSTETRICS & GYNAECOLOGY
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.description.doi10.1210/en.2018-01020
dc.description.sourcetitleENDOCRINOLOGY
dc.description.volume160
dc.description.issue6
dc.description.page1394-1408
dc.published.statePublished
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