Please use this identifier to cite or link to this item: https://doi.org/10.1021/acs.analchem.7b03694
Title: Aggregation-Induced Emission Probe for Specific Turn-On Quantification of Soluble Transferrin Receptor: An Important Disease Marker for Iron Deficiency Anemia and Kidney Diseases
Authors: Zhang, Ruoyu 
Sung, Simon HP
Feng, Guangxue 
Zhang, Chong-Jing 
Kenry 
Tang, Ben Zhong
Liu, Bin 
Keywords: Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
LIGHT-UP PROBE
GUIDED PHOTODYNAMIC ABLATION
SERUM
ASSAY
BIOMARKER
FERRITIN
PROTEOMICS
APOPTOSIS
BIOPROBE
HITACHI
Issue Date: 16-Jan-2018
Publisher: AMERICAN CHEMICAL SOCIETY
Citation: Zhang, Ruoyu, Sung, Simon HP, Feng, Guangxue, Zhang, Chong-Jing, Kenry, Tang, Ben Zhong, Liu, Bin (2018-01-16). Aggregation-Induced Emission Probe for Specific Turn-On Quantification of Soluble Transferrin Receptor: An Important Disease Marker for Iron Deficiency Anemia and Kidney Diseases. ANALYTICAL CHEMISTRY 90 (2) : 1154-1160. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.analchem.7b03694
Abstract: © 2017 American Chemical Society. Transferrin receptor (TfR) is overexpressed on the surface of many cancer cells due to its vital roles in iron circulation and cellular respiration. Soluble transferrin receptor (sTfR), a truncated extracellular form of TfR in serum, is an important marker of iron deficiency anemia (IDA) and bone marrow failure in cancer patients. More recently, sTfR level in urine has been related to a specific kidney disease of Henoch-Schönlein purpura nephritis (HSPN). Despite the universal significance of sTfR, there is still a lack of a simple and sensitive method for the quantification of sTfR. Furthermore, it is desirable to have a probe that can detect both TfR and sTfR for further comparison study. In this work, we developed a water-soluble AIE-peptide conjugate with aggregation-induced emission (AIE) characteristics. Taking advantage of the negligible emission from molecularly dissolved tetraphenylethene (TPE), probe TPE-2T7 was used for the light-up detection of sTfR. The probe itself is nonemissive in aqueous solution, but it turns on its fluorescence upon interaction with sTfR to yield a detection limit of 0.27 μg/mL, which is much lower than the sTfR level in IDA patients. Furthermore, a proof-of-concept experiment validates the potential of the probe for diagnosis of HSPN by urine test.
Source Title: ANALYTICAL CHEMISTRY
URI: https://scholarbank.nus.edu.sg/handle/10635/169839
ISSN: 00032700
15206882
DOI: 10.1021/acs.analchem.7b03694
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