Please use this identifier to cite or link to this item: https://doi.org/10.1002/adfm.201808365
Title: NIR-II Excitable Conjugated Polymer Dots with Bright NIR-I Emission for Deep In Vivo Two-Photon Brain Imaging Through Intact Skull
Authors: Wang, Shaowei 
Liu, Jie
Feng, Guangxue 
Ng, Lai Guan
Liu, Bin 
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
brain imaging
conjugated polymer
deep tissue
NIR-II excitation
two-photon
BONE-MARROW
LONG-TERM
1040 NM
TISSUE
NANOPARTICLES
MICROSCOPY
ABSORPTION
WINDOW
MULTIPHOTON
RESOLUTION
Issue Date: 11-Apr-2019
Publisher: WILEY-V C H VERLAG GMBH
Citation: Wang, Shaowei, Liu, Jie, Feng, Guangxue, Ng, Lai Guan, Liu, Bin (2019-04-11). NIR-II Excitable Conjugated Polymer Dots with Bright NIR-I Emission for Deep In Vivo Two-Photon Brain Imaging Through Intact Skull. ADVANCED FUNCTIONAL MATERIALS 29 (15). ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.201808365
Abstract: © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Methods for noninvasive brain imaging are highly desirable to study brain structures in neuroscience. Two-photon fluorescence microscopy (2PFM) with near-infrared (NIR) light excitation is a relatively noninvasive approach commonly used to study brain with high spatial resolution and large imaging depth. However, most of the current studies require cranial window implantation or skull-thinning methods due to attenuation of excitation light. 2PFM through intact mouse skull is challenging due to strong scattering induced by skull bone. Herein, NIR-II light excitable single-chain conjugated polymer dots (CPdots) with bright fluorescence in NIR-I region (peak at ≈725 nm and quantum yield of 20.6 ± 1.0%) are developed for deep in vivo two-photon fluorescence (2PF) imaging of intact mouse brain. The synthesized CPdots exhibit good biocompatibility, high photostability, and large two-photon absorption cross section. The CPdots allow 2PF images acquired upon excitation at 800, 1040 and 1200 nm with the highest signal-to-background ratio of 208 demonstrated for 1200 nm excitation. Moreover, a 3D reconstruction of the brain blood vessel network is obtained with a large vertical depth of 400 µm through intact skull. This work demonstrates great potential of bright NIR fluorophores for in vivo deep tissue imaging.
Source Title: ADVANCED FUNCTIONAL MATERIALS
URI: https://scholarbank.nus.edu.sg/handle/10635/169620
ISSN: 1616301X
16163028
DOI: 10.1002/adfm.201808365
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