Please use this identifier to cite or link to this item: https://doi.org/10.1002/adfm.201808365
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dc.titleNIR-II Excitable Conjugated Polymer Dots with Bright NIR-I Emission for Deep In Vivo Two-Photon Brain Imaging Through Intact Skull
dc.contributor.authorWang, Shaowei
dc.contributor.authorLiu, Jie
dc.contributor.authorFeng, Guangxue
dc.contributor.authorNg, Lai Guan
dc.contributor.authorLiu, Bin
dc.date.accessioned2020-06-10T07:12:09Z
dc.date.available2020-06-10T07:12:09Z
dc.date.issued2019-04-11
dc.identifier.citationWang, Shaowei, Liu, Jie, Feng, Guangxue, Ng, Lai Guan, Liu, Bin (2019-04-11). NIR-II Excitable Conjugated Polymer Dots with Bright NIR-I Emission for Deep In Vivo Two-Photon Brain Imaging Through Intact Skull. ADVANCED FUNCTIONAL MATERIALS 29 (15). ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.201808365
dc.identifier.issn1616301X
dc.identifier.issn16163028
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/169620
dc.description.abstract© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Methods for noninvasive brain imaging are highly desirable to study brain structures in neuroscience. Two-photon fluorescence microscopy (2PFM) with near-infrared (NIR) light excitation is a relatively noninvasive approach commonly used to study brain with high spatial resolution and large imaging depth. However, most of the current studies require cranial window implantation or skull-thinning methods due to attenuation of excitation light. 2PFM through intact mouse skull is challenging due to strong scattering induced by skull bone. Herein, NIR-II light excitable single-chain conjugated polymer dots (CPdots) with bright fluorescence in NIR-I region (peak at ≈725 nm and quantum yield of 20.6 ± 1.0%) are developed for deep in vivo two-photon fluorescence (2PF) imaging of intact mouse brain. The synthesized CPdots exhibit good biocompatibility, high photostability, and large two-photon absorption cross section. The CPdots allow 2PF images acquired upon excitation at 800, 1040 and 1200 nm with the highest signal-to-background ratio of 208 demonstrated for 1200 nm excitation. Moreover, a 3D reconstruction of the brain blood vessel network is obtained with a large vertical depth of 400 µm through intact skull. This work demonstrates great potential of bright NIR fluorophores for in vivo deep tissue imaging.
dc.language.isoen
dc.publisherWILEY-V C H VERLAG GMBH
dc.sourceElements
dc.subjectScience & Technology
dc.subjectPhysical Sciences
dc.subjectTechnology
dc.subjectChemistry, Multidisciplinary
dc.subjectChemistry, Physical
dc.subjectNanoscience & Nanotechnology
dc.subjectMaterials Science, Multidisciplinary
dc.subjectPhysics, Applied
dc.subjectPhysics, Condensed Matter
dc.subjectChemistry
dc.subjectScience & Technology - Other Topics
dc.subjectMaterials Science
dc.subjectPhysics
dc.subjectbrain imaging
dc.subjectconjugated polymer
dc.subjectdeep tissue
dc.subjectNIR-II excitation
dc.subjecttwo-photon
dc.subjectBONE-MARROW
dc.subjectLONG-TERM
dc.subject1040 NM
dc.subjectTISSUE
dc.subjectNANOPARTICLES
dc.subjectMICROSCOPY
dc.subjectABSORPTION
dc.subjectWINDOW
dc.subjectMULTIPHOTON
dc.subjectRESOLUTION
dc.typeArticle
dc.date.updated2020-06-10T06:20:14Z
dc.contributor.departmentDEPT OF CHEMICAL & BIOMOLECULAR ENGG
dc.description.doi10.1002/adfm.201808365
dc.description.sourcetitleADVANCED FUNCTIONAL MATERIALS
dc.description.volume29
dc.description.issue15
dc.published.statePublished
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