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Title: | NMR Structure and IgE Epitopes of Blo t 21, a Major Dust Mite Allergen from Blomia tropicalis | Authors: | Tan, Kang Wei Ong, Tan Ching Gao, Yun Feng Tiong, Yuen Sung Wong, Kang Ning Chew, Fook Tim Mok, Yu Keung |
Keywords: | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology CROSS-REACTIVITY CHEMICAL-SHIFT P 5 IDENTIFICATION RESONANCE SYSTEM C-13 RESTRAINTS ASSIGNMENT DATABASE |
Issue Date: | 5-Oct-2012 | Publisher: | American Society for Biochemistry and Molecular Biology | Citation: | Tan, Kang Wei, Ong, Tan Ching, Gao, Yun Feng, Tiong, Yuen Sung, Wong, Kang Ning, Chew, Fook Tim, Mok, Yu Keung (2012-10-05). NMR Structure and IgE Epitopes of Blo t 21, a Major Dust Mite Allergen from Blomia tropicalis. JOURNAL OF BIOLOGICAL CHEMISTRY 287 (41) : 34776-34785. ScholarBank@NUS Repository. | Abstract: | Blo t 21 is a paralogue of the group 5 allergen, Blo t 5, a major allergen from the dust mite Blomia tropicalis. Blo t 21 has moderate sequence identity (40.7%) to Blo t 5 and low to moderate cross-reactivity to Blo t 5. In B. tropicalis, the most prevalent and allergenic allergens are in the order of Blo t 21, Blo t 5, and Blo t 7. Here, we determined the NMR solution structure of Blo t 21, which represents the first structure of the group 21 dust mite allergen. The structure of Blo t 21 closely resembles the structures of Blo t 5 and Der p 5, comprising three anti-parallel α-helices arranged in a helical bundle. Using site-directed mutagenesis and specific IgE binding ELISA, Blo t 21 was found to contain both conserved and unique charged IgE epitope residues at the L2 loop region and on helix α3. Cross-inhibition assays confirmed that Blo t 21 has a low to moderate cross-reactivity with Blo t 5 and Der p 5 and represents a novel group of major allergen in B. tropicalis. In addition to group 5 allergens, Blo t 21 has also a low to moderate cross-reactivity with group 21 allergens from Dermatophagoides mites, confirming that B. tropicalis is a major and distinct source of dust mite allergens. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. | Source Title: | JOURNAL OF BIOLOGICAL CHEMISTRY | URI: | https://scholarbank.nus.edu.sg/handle/10635/168892 | ISSN: | 00219258 1083351X |
Appears in Collections: | Elements Staff Publications |
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Blot 21 paper final version.pdf | Published version | 2.01 MB | Adobe PDF | OPEN | Published | View/Download |
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