Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/168892
DC Field | Value | |
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dc.title | NMR Structure and IgE Epitopes of Blo t 21, a Major Dust Mite Allergen from Blomia tropicalis | |
dc.contributor.author | Tan, Kang Wei | |
dc.contributor.author | Ong, Tan Ching | |
dc.contributor.author | Gao, Yun Feng | |
dc.contributor.author | Tiong, Yuen Sung | |
dc.contributor.author | Wong, Kang Ning | |
dc.contributor.author | Chew, Fook Tim | |
dc.contributor.author | Mok, Yu Keung | |
dc.date.accessioned | 2020-06-01T11:21:13Z | |
dc.date.available | 2020-06-01T11:21:13Z | |
dc.date.issued | 2012-10-05 | |
dc.identifier.citation | Tan, Kang Wei, Ong, Tan Ching, Gao, Yun Feng, Tiong, Yuen Sung, Wong, Kang Ning, Chew, Fook Tim, Mok, Yu Keung (2012-10-05). NMR Structure and IgE Epitopes of Blo t 21, a Major Dust Mite Allergen from Blomia tropicalis. JOURNAL OF BIOLOGICAL CHEMISTRY 287 (41) : 34776-34785. ScholarBank@NUS Repository. | |
dc.identifier.issn | 00219258 | |
dc.identifier.issn | 1083351X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/168892 | |
dc.description.abstract | Blo t 21 is a paralogue of the group 5 allergen, Blo t 5, a major allergen from the dust mite Blomia tropicalis. Blo t 21 has moderate sequence identity (40.7%) to Blo t 5 and low to moderate cross-reactivity to Blo t 5. In B. tropicalis, the most prevalent and allergenic allergens are in the order of Blo t 21, Blo t 5, and Blo t 7. Here, we determined the NMR solution structure of Blo t 21, which represents the first structure of the group 21 dust mite allergen. The structure of Blo t 21 closely resembles the structures of Blo t 5 and Der p 5, comprising three anti-parallel α-helices arranged in a helical bundle. Using site-directed mutagenesis and specific IgE binding ELISA, Blo t 21 was found to contain both conserved and unique charged IgE epitope residues at the L2 loop region and on helix α3. Cross-inhibition assays confirmed that Blo t 21 has a low to moderate cross-reactivity with Blo t 5 and Der p 5 and represents a novel group of major allergen in B. tropicalis. In addition to group 5 allergens, Blo t 21 has also a low to moderate cross-reactivity with group 21 allergens from Dermatophagoides mites, confirming that B. tropicalis is a major and distinct source of dust mite allergens. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. | |
dc.language.iso | en | |
dc.publisher | American Society for Biochemistry and Molecular Biology | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | CROSS-REACTIVITY | |
dc.subject | CHEMICAL-SHIFT | |
dc.subject | P 5 | |
dc.subject | IDENTIFICATION | |
dc.subject | RESONANCE | |
dc.subject | SYSTEM | |
dc.subject | C-13 | |
dc.subject | RESTRAINTS | |
dc.subject | ASSIGNMENT | |
dc.subject | DATABASE | |
dc.type | Article | |
dc.date.updated | 2020-05-28T07:53:00Z | |
dc.contributor.department | BIOLOGY (NU) | |
dc.contributor.department | DEPT OF CHEMISTRY | |
dc.contributor.department | MECHANOBIOLOGY INSTITUTE | |
dc.description.sourcetitle | JOURNAL OF BIOLOGICAL CHEMISTRY | |
dc.description.volume | 287 | |
dc.description.issue | 41 | |
dc.description.page | 34776-34785 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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Blot 21 paper final version.pdf | Published version | 2.01 MB | Adobe PDF | OPEN | Published | View/Download |
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