Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/168892
DC FieldValue
dc.titleNMR Structure and IgE Epitopes of Blo t 21, a Major Dust Mite Allergen from Blomia tropicalis
dc.contributor.authorTan, Kang Wei
dc.contributor.authorOng, Tan Ching
dc.contributor.authorGao, Yun Feng
dc.contributor.authorTiong, Yuen Sung
dc.contributor.authorWong, Kang Ning
dc.contributor.authorChew, Fook Tim
dc.contributor.authorMok, Yu Keung
dc.date.accessioned2020-06-01T11:21:13Z
dc.date.available2020-06-01T11:21:13Z
dc.date.issued2012-10-05
dc.identifier.citationTan, Kang Wei, Ong, Tan Ching, Gao, Yun Feng, Tiong, Yuen Sung, Wong, Kang Ning, Chew, Fook Tim, Mok, Yu Keung (2012-10-05). NMR Structure and IgE Epitopes of Blo t 21, a Major Dust Mite Allergen from Blomia tropicalis. JOURNAL OF BIOLOGICAL CHEMISTRY 287 (41) : 34776-34785. ScholarBank@NUS Repository.
dc.identifier.issn00219258
dc.identifier.issn1083351X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/168892
dc.description.abstractBlo t 21 is a paralogue of the group 5 allergen, Blo t 5, a major allergen from the dust mite Blomia tropicalis. Blo t 21 has moderate sequence identity (40.7%) to Blo t 5 and low to moderate cross-reactivity to Blo t 5. In B. tropicalis, the most prevalent and allergenic allergens are in the order of Blo t 21, Blo t 5, and Blo t 7. Here, we determined the NMR solution structure of Blo t 21, which represents the first structure of the group 21 dust mite allergen. The structure of Blo t 21 closely resembles the structures of Blo t 5 and Der p 5, comprising three anti-parallel α-helices arranged in a helical bundle. Using site-directed mutagenesis and specific IgE binding ELISA, Blo t 21 was found to contain both conserved and unique charged IgE epitope residues at the L2 loop region and on helix α3. Cross-inhibition assays confirmed that Blo t 21 has a low to moderate cross-reactivity with Blo t 5 and Der p 5 and represents a novel group of major allergen in B. tropicalis. In addition to group 5 allergens, Blo t 21 has also a low to moderate cross-reactivity with group 21 allergens from Dermatophagoides mites, confirming that B. tropicalis is a major and distinct source of dust mite allergens. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.language.isoen
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiochemistry & Molecular Biology
dc.subjectCROSS-REACTIVITY
dc.subjectCHEMICAL-SHIFT
dc.subjectP 5
dc.subjectIDENTIFICATION
dc.subjectRESONANCE
dc.subjectSYSTEM
dc.subjectC-13
dc.subjectRESTRAINTS
dc.subjectASSIGNMENT
dc.subjectDATABASE
dc.typeArticle
dc.date.updated2020-05-28T07:53:00Z
dc.contributor.departmentBIOLOGY (NU)
dc.contributor.departmentDEPT OF CHEMISTRY
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.description.sourcetitleJOURNAL OF BIOLOGICAL CHEMISTRY
dc.description.volume287
dc.description.issue41
dc.description.page34776-34785
dc.published.statePublished
Appears in Collections:Elements
Staff Publications

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Blot 21 paper final version.pdfPublished version2.01 MBAdobe PDF

OPEN

PublishedView/Download

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.