Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep18293
Title: DNA-Directed Assembly of Nanogold Dimers: A Unique Dynamic Light Scattering Sensing Probe for Transcription Factor Detection
Authors: Seow, Nianjia 
Tan, Yen Nee 
Yung, Lin-Yue Lanry 
Su, Xiaodi 
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
GOLD NANOPARTICLES
LABEL-FREE
PROTEIN
CANCER
ASSAY
AGGREGATION
RECEPTORS
BIOSENSOR
Issue Date: 18-Dec-2015
Publisher: NATURE PUBLISHING GROUP
Citation: Seow, Nianjia, Tan, Yen Nee, Yung, Lin-Yue Lanry, Su, Xiaodi (2015-12-18). DNA-Directed Assembly of Nanogold Dimers: A Unique Dynamic Light Scattering Sensing Probe for Transcription Factor Detection. SCIENTIFIC REPORTS 5 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/srep18293
Abstract: We have developed a unique DNA-assembled gold nanoparticles (AuNPs) dimer for dynamic light scattering (DLS) sensing of transcription factors, exemplified by estrogen receptor (ER) that binds specifically to a double-stranded (ds) DNA sequence containing estrogen response element (ERE). Here, ERE sequence is incorporated into the DNA linkers to bridge the AuNPs dimer for ER binding. Coupled with DLS, this AuNP dimer-based DLS detection system gave distinct readout of a single â € complex peakâ €™ in the presence of the target molecule (i.e., ER). This unique signature marked the first time that such nanostructures can be used to study transcription factor-DNA interactions, which DLS alone cannot do. This was also unlike previously reported AuNP-DLS assays that gave random and broad distribution of particles size upon target binding. In addition, the ERE-containing AuNP dimers could also suppress the light-scattering signal from the unbound proteins and other interfering factors (e.g., buffer background), and has potential for sensitive detection of target proteins in complex biological samples such as cell lysates. In short, the as-developed AuNP dimer probe coupled with DLS is a simple (mix and test), rapid (readout in ∼5 min) and sensitive (low nM levels of ER) platform to detect sequence-specific protein-DNA binding event.
Source Title: SCIENTIFIC REPORTS
URI: https://scholarbank.nus.edu.sg/handle/10635/168696
ISSN: 20452322
20452322
DOI: 10.1038/srep18293
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