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Title: Blue Light-Directed Cell Migration, Aggregation, and Patterning
Authors: Zhang, J 
Luo, Y
Poh, CL 
Keywords: directional motility
strain separation
synthetic gene circuits
Issue Date: 1-Jan-2020
Publisher: Elsevier BV
Citation: Zhang, J, Luo, Y, Poh, CL (2020-01-01). Blue Light-Directed Cell Migration, Aggregation, and Patterning. Journal of Molecular Biology. ScholarBank@NUS Repository.
Abstract: © 2020 Elsevier Ltd Bacterial motility is related to many cellular activities, such as cell migration, aggregation, and biofilm formations. The ability to control motility and direct the bacteria to certain location could be used to guide the bacteria in applications such as seeking for and killing pathogen, forming various population-level patterns, and delivering of drugs and vaccines. Currently, bacteria motility is mainly controlled by chemotaxis (prescribed chemical stimuli), which needs physical contact with the chemical inducer. This lacks the flexibility for pattern formation as it has limited spatial control. To overcome the limitations, we developed blue light-regulated synthetic genetic circuit to control bacterial directional motility, by taking the advantage that light stimulus can be delivered to cells in different patterns with precise spatial control. The circuit developed enables programmed Escherichia coli cells to increase directional motility and move away from the blue light, i.e., that negative phototaxis is utilized. This further allows the control of the cells to form aggregation with different patterns. Further, we showed that the circuit can be used to separate two different strains. The demonstrated ability of blue light-controllable gene circuits to regulate a CheZ expression could give researchers more means to control bacterial motility and pattern formation.
Source Title: Journal of Molecular Biology
ISSN: 00222836
DOI: 10.1016/j.jmb.2020.03.029
Appears in Collections:Staff Publications

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