Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biotechadv.2018.07.005
Title: Synthetic biology toolkits and applications in Saccharomyces cerevisiae
Authors: CHEN BINBIN 
LEE HUI LING 
HENG YU CHYUAN 
CHUA NIYING 
TEO WEI SUONG 
CHOI WON JAE 
SUSANNA LEONG SU JAN 
FOO JEE LOON 
Chang,Matthew Wook 
Keywords: Yeast
Saccharomyces cerevisiae
Synthetic biology
Abstraction
Genetic toolkits
Applications
Issue Date: 18-Jul-2018
Citation: CHEN BINBIN, LEE HUI LING, HENG YU CHYUAN, CHUA NIYING, TEO WEI SUONG, CHOI WON JAE, SUSANNA LEONG SU JAN, FOO JEE LOON, Chang,Matthew Wook (2018-07-18). Synthetic biology toolkits and applications in Saccharomyces cerevisiae. Biotechnology advances 36 (7) : 1870-1881. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biotechadv.2018.07.005
Abstract: Synthetic biologists construct biological components and systems to look into biological phenomena and drive a myriad of practical applications that aim to tackle current global challenges in energy, healthcare and the environment. While most tools have been established in bacteria, particularly Escherichia coli, recent years have seen parallel developments in the model yeast strain Saccharomyces cerevisiae, one of the most well-understood eukaryotic biological system. Here, we outline the latest advances in yeast synthetic biology tools based on a framework of abstraction hierarchies of parts, circuits and genomes. In brief, the creation and characterization of biological parts are explored at the transcriptional, translational and post-translational levels. Using characterized parts as building block units, the designing of functional circuits is elaborated with examples. In addition, the status and potential applications of synthetic genomes as a genome level platform for biological system construction are also discussed. In addition to the development of a toolkit, we describe how those tools have been applied in the areas of drug production and screening, study of disease mechanisms, pollutant sensing and bioremediation. Finally, we provide a future outlook of yeast as a workhorse of eukaryotic genetics and a chosen chassis in this field.
Source Title: Biotechnology advances
URI: https://scholarbank.nus.edu.sg/handle/10635/167867
ISSN: 07349750
DOI: 10.1016/j.biotechadv.2018.07.005
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