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Title: Hydrogen Sulfide Suppresses Outward Rectifier Potassium Currents in Human Pluripotent Stem Cell-Derived Cardiomyocytes
Authors: Wei H. 
Zhang G.
Qiu S.
Lu J.
Sheng J.
Tan G.
Wong P.
Gan S.U. 
Shim W. 
Keywords: calcium channel L type
hydrogen sulfide
outward rectifier potassium channel
potassium channel
unclassified drug
calcium cell level
cell differentiation
cell function
channel gating
concentration response
controlled study
embryonic stem cell
heart electrophysiology
heart muscle cell
heart muscle fiber membrane potential
human cell
molecular imaging
molecular interaction
patch clamp
pluripotent stem cell
Action Potentials
Calcium Channel Blockers
Calcium Channels, L-Type
Cell Differentiation
Electric Conductivity
Embryonic Stem Cells
Heart Atria
Heart Ventricles
Hydrogen Sulfide
Myocytes, Cardiac
Pluripotent Stem Cells
Potassium Channel Blockers
Potassium Channels
Issue Date: 2012
Publisher: Public Library of Science
Citation: Wei H., Zhang G., Qiu S., Lu J., Sheng J., Manasi, Tan G., Wong P., Gan S.U., Shim W. (2012). Hydrogen Sulfide Suppresses Outward Rectifier Potassium Currents in Human Pluripotent Stem Cell-Derived Cardiomyocytes. PLoS ONE 7 (11) : e50641. ScholarBank@NUS Repository.
Abstract: Aim: Hydrogen sulfide (H2S) is a promising cardioprotective agent and a potential modulator of cardiac ion currents. Yet its cardiac effects on humans are poorly understood due to lack of functional cardiomyocytes. This study investigates electrophysiological responses of human pluripotent stem cells (hPSCs) derived cardiomyocytes towards H2S. Methods and Results: Cardiomyocytes of ventricular, atrial and nodal subtypes differentiated from H9 embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were electrophysiologically characterized. The effect of NaHS, a donor of H2S, on action potential (AP), outward rectifier potassium currents (IKs and IKr), L-type Ca2+ currents (ICaL) and hyperpolarization-activated inward current (If) were determined by patch-clamp electrophysiology and confocal calcium imaging. In a concentration-dependent manner, NaHS (100 to 300 ?M) consistently altered the action potential properties including prolonging action potential duration (APD) and slowing down contracting rates of ventricular-and atrial-like cardiomyocytes derived from both hESCs and hiPSCs. Moreover, inhibitions of slow and rapid IK (IKs and IKr), ICaL and If were found in NaHS treated cardiomyocytes and it could collectively contribute to the remodeling of AP properties. Conclusions: This is the first demonstration of effects of H2S on cardiac electrophysiology of human ventricular-like, atrial-like and nodal-like cardiomyocytes. It reaffirmed the inhibitory effect of H2S on ICaL and revealed additional novel inhibitory effects on If, IKs and IKr currents in human cardiomyocytes. © 2012 Wei et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0050641
Appears in Collections:Staff Publications

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