Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0201768
Title: Human pharyngeal microbiota in age-related macular degeneration
Authors: Ho E.X.P.
Cheung C.M.G. 
Sim S.
Chu C.W.
Wilm A.
Lin C.B.
Mathur R.
Wong D.
Chan C.M.
Bhagarva M.
Laude A.
Lim T.H.
Wong T.Y. 
Cheng C.Y. 
Davila S.
Hibberd M. 
Keywords: RNA 16S
bacterial RNA
16S ribosomal RNA gene
adult
age related macular degeneration
Article
controlled study
disease association
female
Gemella
high throughput sequencing
human
major clinical study
male
microbial colonization
microflora
middle aged
nasopharynx
nonhuman
pathogenesis
Prevotella
RNA gene
RNA sequence
Singapore
aged
case control study
cohort analysis
genetics
macular degeneration
microbiology
nose cavity
pharynx
very elderly
Adult
Aged
Aged, 80 and over
Case-Control Studies
Cohort Studies
Female
Humans
Macular Degeneration
Male
Microbiota
Middle Aged
Nasal Cavity
Pharynx
RNA, Bacterial
RNA, Ribosomal, 16S
Singapore
Issue Date: 2018
Publisher: Public Library of Science
Citation: Ho E.X.P., Cheung C.M.G., Sim S., Chu C.W., Wilm A., Lin C.B., Mathur R., Wong D., Chan C.M., Bhagarva M., Laude A., Lim T.H., Wong T.Y., Cheng C.Y., Davila S., Hibberd M. (2018). Human pharyngeal microbiota in age-related macular degeneration. PLoS ONE 13 (8) : e0201768. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0201768
Abstract: Background While the aetiology of age-related macular degeneration (AMD)—a major blinding disease —remains unknown, the disease is strongly associated with variants in the complement factor H (CFH) gene. CFH variants also confer susceptibility to invasive infection with several bacterial colonizers of the nasopharyngeal mucosa. This shared susceptibility locus implicates complement deregulation as a common disease mechanism, and suggests the possibility that microbial interactions with host complement may trigger AMD. In this study, we address this possibility by testing the hypothesis that AMD is associated with specific microbial colonization of the human nasopharynx. Results High-throughput Illumina sequencing of the V3-V6 region of the microbial 16S ribosomal RNA gene was used to comprehensively and accurately describe the human pharyngeal microbiome, at genus level, in 245 AMD patients and 386 controls. Based on mean and differential microbial abundance analyses, we determined an overview of the pharyngeal microbiota, as well as candidate genera (Prevotella and Gemella) suggesting an association towards AMD health and disease conditions. Conclusions Utilizing an extensive study population from Singapore, our results provided an accurate description of the pharyngeal microbiota profiles in AMD health and disease conditions. Through identification of candidate genera that are different between conditions, we provide preliminary evidence for the existence of microbial triggers for AMD. Ethical approval for this study was obtained through the Singapore Health Clinical Institutional Review Board, reference numbers R799/63/2010 and 2010/585/A. © 2018 Ho et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/165897
ISSN: 19326203
DOI: 10.1371/journal.pone.0201768
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