Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0103525
Title: Expression profiling of RNA transcripts during neuronal maturation and ischemic injury
Authors: Kaur P. 
Karolina D.S. 
Sepramaniam S. 
Armugam A. 
Jeyaseelan K. 
Keywords: contactin 1
long untranslated RNA
messenger RNA
microRNA
microRNA 124
microRNA 128
microRNA 129 5p
microRNA 203
microRNA 218
microRNA 290 5p
microRNA 326
microRNA 329
microRNA 377
microRNA 495
neurotrophin
transcriptome
unclassified drug
axin
Axin2 protein, mouse
CD56 antigen
Cntn1 protein, mouse
contactin 1
Lnk protein, mouse
messenger RNA
Ncam1 protein, mouse
NEGR1 protein, mouse
nerve cell adhesion molecule
Nrxn1 protein, mouse
prkcb1 protein, mouse
protein kinase C beta
signal peptide
animal cell
animal experiment
animal tissue
article
astrocyte
axin2 gene
axonogenesis
brain cell culture
cell adhesion
cell maturation
cell proliferation
cell survival
Cntn1 gene
controlled study
dendritogenesis
embryo
gene
gene cluster
gene expression profiling
gene expression regulation
gene ontology
gene regulatory network
glia cell
hypoxic ischemic encephalopathy
in vitro study
intracellular signaling
NCAM1 gene
NEGR1 gene
nerve cell
nerve cell differentiation
nerve fiber growth
nerve function
nonhuman
NRXN1 gene
nucleotide sequence
Prkcb gene
RNA sequence
RNA transcription
Sh2b3 gene
synaptogenesis
animal
brain
cell culture
cytology
embryology
genetics
metabolism
mouse
nerve cell
nervous system development
reperfusion injury
vascularization
Animals
Antigens, CD56
Axin Protein
Brain
Cell Adhesion Molecules, Neuronal
Cells, Cultured
Contactin 1
Gene Expression Regulation, Developmental
Intracellular Signaling Peptides and Proteins
Mice
Neural Cell Adhesion Molecules
Neurogenesis
Neurons
Protein Kinase C beta
Reperfusion Injury
RNA, Messenger
Issue Date: 2014
Publisher: Public Library of Science
Citation: Kaur P., Karolina D.S., Sepramaniam S., Armugam A., Jeyaseelan K. (2014). Expression profiling of RNA transcripts during neuronal maturation and ischemic injury. PLoS ONE 9 (7) : e103525. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0103525
Abstract: Neuronal development is a pro-survival process that involves neurite growth, synaptogenesis, synaptic and neuronal pruning. During development, these processes can be controlled by temporal gene expression that is orchestrated by both long non-coding RNAs and microRNAs. To examine the interplay between these different components of the transcriptome during neuronal differentiation, we carried out mRNA, long non-coding RNA and microRNA expression profiling on maturing primary neurons. Subsequent gene ontology analysis revealed regulation of axonogenesis and dendritogenesis processes by these differentially expressed mRNAs and non-coding RNAs. Temporally regulated mRNAs and their associated long non-coding RNAs were significantly over-represented in proliferation and differentiation associated signalling, cell adhesion and neurotrophin signalling pathways. Verification of expression of the Axin2, Prkcb, Cntn1, Ncam1, Negr1, Nrxn1 and Sh2b3 mRNAs and their respective long non-coding RNAs in an in vitro model of ischemic-reperfusion injury showed an inverse expression profile to the maturation process, thus suggesting their role(s) in maintaining neuronal structure and function. Furthermore, we propose that expression of the cell adhesion molecules, Ncam1 and Negr1 might be tightly regulated by both long non-coding RNAs and microRNAs. © 2014 Kaur et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/165710
ISSN: 19326203
DOI: 10.1371/journal.pone.0103525
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