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Title: Discovery of molecular markers to discriminate corneal endothelial cells in the human body
Authors: Masahito Y.
Hiroko O.
Susumu H.
Satoshi K.
Yoshihide H.
Masayoshi I.
Hideya K.
Motokazu T.
Kohji N.
Keywords: molecular marker
biological marker
carrier protein
CLRN1 protein, human
egg protein
G protein coupled receptor
GRIP1 protein, human
membrane protein
MRGPRX3 protein, human
nerve protein
serotonin 1D receptor
zona pellucida glycoprotein
ZP4 protein, human
cell function
cell level
cell population
cell specificity
cellular distribution
CLRN1 gene
computer program
controlled study
cornea endothelium
corneal endothelial cell
data analysis
endothelium cell
gene expression
genetic marker
GRIP1 gene
HTR1D gene
human cell
human tissue
marker gene
MRGPRX3 gene
RNA sequence
sequence database
ZP4 gene
cornea endothelium
cornea transplantation
endothelium cell
middle aged
tissue engineering
Carrier Proteins
Corneal Transplantation
Egg Proteins
Endothelial Cells
Endothelium, Corneal
Membrane Glycoproteins
Membrane Proteins
Middle Aged
Nerve Tissue Proteins
Receptor, Serotonin, 5-HT1D
Receptors, G-Protein-Coupled
Tissue Engineering
Zona Pellucida Glycoproteins
Issue Date: 2015
Publisher: Public Library of Science
Citation: Masahito Y., Hiroko O., Susumu H., Satoshi K., Yoshihide H., Masayoshi I., Hideya K., Motokazu T., Kohji N. (2015). Discovery of molecular markers to discriminate corneal endothelial cells in the human body. PLoS ONE 10 (3) : e0117581. ScholarBank@NUS Repository.
Abstract: The corneal endothelium is a monolayer of hexagonal corneal endothelial cells (CECs) on the inner surface of the cornea. CECs are critical in maintaining corneal transparency through their barrier and pump functions. CECs in vivo have a limited capacity in proliferation, and loss of a significant number of CECs results in corneal edema called bullous keratopathy which can lead to severe visual loss. Corneal transplantation is the most effective method to treat corneal endothelial dysfunction, where it suffers from donor shortage. Therefore, regeneration of CECs from other cell types attracts increasing interests, and specific markers of CECs are crucial to identify actual CECs. However, the currently used markers are far from satisfactory because of their non-specific expression in other cell types. Here, we explored molecular markers to discriminate CECs from other cell types in the human body by integrating the published RNA-seq data of CECs and the FANTOM5 atlas representing diverse range of cell types based on expression patterns. We identified five genes, CLRN1, MRGPRX3, HTR1D, GRIP1 and ZP4 as novel markers of CECs, and the specificities of these genes were successfully confirmed by independent experiments at both the RNA and protein levels. Notably none of them have been documented in the context of CEC function. These markers could be useful for the purification of actual CECs, and also available for the evaluation of the products derived from other cell types. Our results demonstrate an effective approach to identify molecular markers for CECs and open the door for the regeneration of CECs in vitro. © 2015 Yoshihara et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0117581
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