Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0117581
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dc.title | Discovery of molecular markers to discriminate corneal endothelial cells in the human body | |
dc.contributor.author | Masahito Y. | |
dc.contributor.author | Hiroko O. | |
dc.contributor.author | Susumu H. | |
dc.contributor.author | Satoshi K. | |
dc.contributor.author | Yoshihide H. | |
dc.contributor.author | Masayoshi I. | |
dc.contributor.author | Hideya K. | |
dc.contributor.author | Motokazu T. | |
dc.contributor.author | Kohji N. | |
dc.date.accessioned | 2020-03-19T03:02:40Z | |
dc.date.available | 2020-03-19T03:02:40Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Masahito Y., Hiroko O., Susumu H., Satoshi K., Yoshihide H., Masayoshi I., Hideya K., Motokazu T., Kohji N. (2015). Discovery of molecular markers to discriminate corneal endothelial cells in the human body. PLoS ONE 10 (3) : e0117581. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0117581 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/165700 | |
dc.description.abstract | The corneal endothelium is a monolayer of hexagonal corneal endothelial cells (CECs) on the inner surface of the cornea. CECs are critical in maintaining corneal transparency through their barrier and pump functions. CECs in vivo have a limited capacity in proliferation, and loss of a significant number of CECs results in corneal edema called bullous keratopathy which can lead to severe visual loss. Corneal transplantation is the most effective method to treat corneal endothelial dysfunction, where it suffers from donor shortage. Therefore, regeneration of CECs from other cell types attracts increasing interests, and specific markers of CECs are crucial to identify actual CECs. However, the currently used markers are far from satisfactory because of their non-specific expression in other cell types. Here, we explored molecular markers to discriminate CECs from other cell types in the human body by integrating the published RNA-seq data of CECs and the FANTOM5 atlas representing diverse range of cell types based on expression patterns. We identified five genes, CLRN1, MRGPRX3, HTR1D, GRIP1 and ZP4 as novel markers of CECs, and the specificities of these genes were successfully confirmed by independent experiments at both the RNA and protein levels. Notably none of them have been documented in the context of CEC function. These markers could be useful for the purification of actual CECs, and also available for the evaluation of the products derived from other cell types. Our results demonstrate an effective approach to identify molecular markers for CECs and open the door for the regeneration of CECs in vitro. © 2015 Yoshihara et al. | |
dc.publisher | Public Library of Science | |
dc.source | Unpaywall 20200320 | |
dc.subject | molecular marker | |
dc.subject | biological marker | |
dc.subject | carrier protein | |
dc.subject | CLRN1 protein, human | |
dc.subject | egg protein | |
dc.subject | G protein coupled receptor | |
dc.subject | GRIP1 protein, human | |
dc.subject | membrane protein | |
dc.subject | MRGPRX3 protein, human | |
dc.subject | nerve protein | |
dc.subject | serotonin 1D receptor | |
dc.subject | zona pellucida glycoprotein | |
dc.subject | ZP4 protein, human | |
dc.subject | adult | |
dc.subject | Article | |
dc.subject | cell function | |
dc.subject | cell level | |
dc.subject | cell population | |
dc.subject | cell specificity | |
dc.subject | cellular distribution | |
dc.subject | CLRN1 gene | |
dc.subject | computer program | |
dc.subject | controlled study | |
dc.subject | cornea endothelium | |
dc.subject | corneal endothelial cell | |
dc.subject | data analysis | |
dc.subject | endothelium cell | |
dc.subject | fetus | |
dc.subject | gene expression | |
dc.subject | genetic marker | |
dc.subject | GRIP1 gene | |
dc.subject | HTR1D gene | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | human tissue | |
dc.subject | marker gene | |
dc.subject | MRGPRX3 gene | |
dc.subject | RNA sequence | |
dc.subject | sequence database | |
dc.subject | ZP4 gene | |
dc.subject | cornea | |
dc.subject | cornea endothelium | |
dc.subject | cornea transplantation | |
dc.subject | cytology | |
dc.subject | endothelium cell | |
dc.subject | metabolism | |
dc.subject | middle aged | |
dc.subject | physiology | |
dc.subject | procedures | |
dc.subject | regeneration | |
dc.subject | tissue engineering | |
dc.subject | Biomarkers | |
dc.subject | Carrier Proteins | |
dc.subject | Cornea | |
dc.subject | Corneal Transplantation | |
dc.subject | Egg Proteins | |
dc.subject | Endothelial Cells | |
dc.subject | Endothelium, Corneal | |
dc.subject | Humans | |
dc.subject | Membrane Glycoproteins | |
dc.subject | Membrane Proteins | |
dc.subject | Middle Aged | |
dc.subject | Nerve Tissue Proteins | |
dc.subject | Receptor, Serotonin, 5-HT1D | |
dc.subject | Receptors, G-Protein-Coupled | |
dc.subject | Regeneration | |
dc.subject | Tissue Engineering | |
dc.subject | Zona Pellucida Glycoproteins | |
dc.type | Article | |
dc.contributor.department | OPHTHALMOLOGY | |
dc.description.doi | 10.1371/journal.pone.0117581 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 10 | |
dc.description.issue | 3 | |
dc.description.page | e0117581 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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