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https://doi.org/10.1371/journal.pone.0039320
Title: | 92-gene molecular profiling in identification of cancer origin: A retrospective study in Chinese population and performance within different subgroups | Authors: | Wu F. Huang D. Wang L. Xu Q. Liu F. Ye X. Meng X. Du X. |
Keywords: | adrenal cancer article bladder cancer breast cancer cancer diagnosis cancer grading Chinese controlled study diagnostic accuracy diagnostic procedure diagnostic test accuracy study endometrium cancer female gallbladder cancer gastrointestinal stromal tumor gene expression profiling germ cell tumor head and neck cancer human human cell human tissue intestine cancer kidney cancer laser capture microdissection liver cancer lung cancer lymphoma major clinical study male malignant neoplastic disease melanoma mesothelioma metastasis neuroendocrine tumor ovary cancer pancreas cancer performance measurement system population research predictive value primary tumor prostate cancer reference value retrospective study sarcoma scoring system sensitivity and specificity skin cancer thyroid cancer Adult Asian Continental Ancestry Group China Female Gene Expression Profiling Humans Male Neoplasms Retrospective Studies Sensitivity and Specificity |
Issue Date: | 2012 | Publisher: | Public Library of Science | Citation: | Wu F., Huang D., Wang L., Xu Q., Liu F., Ye X., Meng X., Du X. (2012). 92-gene molecular profiling in identification of cancer origin: A retrospective study in Chinese population and performance within different subgroups. PLoS ONE 7 (6) : e39320. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0039320 | Abstract: | Background: After cancer diagnosis, therapy for the patient is largely dependent on the tumor origin, especially when a metastatic tumor is being treated. However, cases such as untypical metastasis, poorly differentiated tumors or even a limited number of tumor cells may lead to challenges in identifying the origin. Moreover, approximately 3% to 5% of total solid tumor patients will not have to have their tumor origin identified in their lifetime. The THEROS CancerTYPE ID® is designed for identifying the tumor origin with an objective, rapid and standardized procedure. Methodology and Principal Findings: This is a blinded retrospective study to evaluate performance of the THEROS CancerTYPE ID® in a Chinese population. In total, 184 formalin-fixed paraffin-embedded (FFPE) samples of 23 tumor origins were collected from the tissue bank of Fudan University Shanghai Cancer Center (FDUSCC). A standard tumor cell enrichment process was used, and the prediction results were compared with reference diagnosis, which was confirmed by two experienced pathologists at FDUSCC. All of the 184 samples were successfully analyzed, and no tumor specimens were excluded because of sample quality issues. In total, 151 samples were correctly predicted. The agreement rate was 82.1%. A Pearson Chi-square test shows that there is no difference between this study and the previous evaluation test performed by bioTheranostics Inc. No statistically significant decrease was observed in either the metastasis group or tumors with high grades. Conclusions: A comparable result with previous work was obtained. Specifically, specimens with a high probability score (>0.85) have a high chance (agreement rate = 95%) of being correctly predicted. No performance difference was observed between primary and metastatic specimens, and no difference was observed among three tumor grades. The use of laser capture micro-dissection (LCM) makes the THEROS CancerTYPE ID® accessible to almost all of the cancer patients with different tumor statuses. © 2012 Wu et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/165570 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0039320 |
Appears in Collections: | Elements Staff Publications |
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