Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1005704
Title: Retromer Is Essential for Autophagy-Dependent Plant Infection by the Rice Blast Fungus
Authors: Zheng W.
Zhou J.
He Y.
Xie Q.
Chen A.
Zheng H.
Shi L.
Zhao X.
Zhang C.
Huang Q.
Fang K.
Lu G.
Ebbole D.J.
Li G.
Naqvi N.I. 
Wang Z.
Keywords: fat droplet
glycogen
amino acid sequence
autophagy
cell vacuole
genetics
host pathogen interaction
Magnaporthe
metabolism
microbiology
Oryza
pathogenicity
plant disease
plant leaf
protein transport
trans Golgi network
Amino Acid Sequence
Autophagy
Glycogen
Host-Pathogen Interactions
Lipid Droplets
Magnaporthe
Oryza
Plant Diseases
Plant Leaves
Protein Transport
trans-Golgi Network
Vacuoles
Issue Date: 2015
Publisher: Public Library of Science
Citation: Zheng W., Zhou J., He Y., Xie Q., Chen A., Zheng H., Shi L., Zhao X., Zhang C., Huang Q., Fang K., Lu G., Ebbole D.J., Li G., Naqvi N.I., Wang Z. (2015). Retromer Is Essential for Autophagy-Dependent Plant Infection by the Rice Blast Fungus. PLoS Genetics 11 (12) : e1005704. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1005704
Abstract: The retromer mediates protein trafficking through recycling cargo from endosomes to the trans-Golgi network in eukaryotes. However, the role of such trafficking events during pathogen-host interaction remains unclear. Here, we report that the cargo-recognition complex (MoVps35, MoVps26 and MoVps29) of the retromer is essential for appressorium-mediated host penetration by Magnaporthe oryzae, the causal pathogen of the blast disease in rice. Loss of retromer function blocked glycogen distribution and turnover of lipid bodies, delayed nuclear degeneration and reduced turgor during appressorial development. Cytological observation revealed dynamic MoVps35-GFP foci co-localized with autophagy-related protein RFP-MoAtg8 at the periphery of autolysosomes. Furthermore, RFP-MoAtg8 interacted with MoVps35-GFP in vivo, RFP-MoAtg8 was mislocalized to the vacuole and failed to recycle from the autolysosome in the absence of the retromer function, leading to impaired biogenesis of autophagosomes. We therefore conclude that retromer is essential for autophagy-dependent plant infection by the rice blast fungus. © 2015 Zheng et al.
Source Title: PLoS Genetics
URI: https://scholarbank.nus.edu.sg/handle/10635/165389
ISSN: 15537390
DOI: 10.1371/journal.pgen.1005704
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