Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1005704
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dc.titleRetromer Is Essential for Autophagy-Dependent Plant Infection by the Rice Blast Fungus
dc.contributor.authorZheng W.
dc.contributor.authorZhou J.
dc.contributor.authorHe Y.
dc.contributor.authorXie Q.
dc.contributor.authorChen A.
dc.contributor.authorZheng H.
dc.contributor.authorShi L.
dc.contributor.authorZhao X.
dc.contributor.authorZhang C.
dc.contributor.authorHuang Q.
dc.contributor.authorFang K.
dc.contributor.authorLu G.
dc.contributor.authorEbbole D.J.
dc.contributor.authorLi G.
dc.contributor.authorNaqvi N.I.
dc.contributor.authorWang Z.
dc.date.accessioned2020-03-13T05:24:05Z
dc.date.available2020-03-13T05:24:05Z
dc.date.issued2015
dc.identifier.citationZheng W., Zhou J., He Y., Xie Q., Chen A., Zheng H., Shi L., Zhao X., Zhang C., Huang Q., Fang K., Lu G., Ebbole D.J., Li G., Naqvi N.I., Wang Z. (2015). Retromer Is Essential for Autophagy-Dependent Plant Infection by the Rice Blast Fungus. PLoS Genetics 11 (12) : e1005704. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1005704
dc.identifier.issn15537390
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/165389
dc.description.abstractThe retromer mediates protein trafficking through recycling cargo from endosomes to the trans-Golgi network in eukaryotes. However, the role of such trafficking events during pathogen-host interaction remains unclear. Here, we report that the cargo-recognition complex (MoVps35, MoVps26 and MoVps29) of the retromer is essential for appressorium-mediated host penetration by Magnaporthe oryzae, the causal pathogen of the blast disease in rice. Loss of retromer function blocked glycogen distribution and turnover of lipid bodies, delayed nuclear degeneration and reduced turgor during appressorial development. Cytological observation revealed dynamic MoVps35-GFP foci co-localized with autophagy-related protein RFP-MoAtg8 at the periphery of autolysosomes. Furthermore, RFP-MoAtg8 interacted with MoVps35-GFP in vivo, RFP-MoAtg8 was mislocalized to the vacuole and failed to recycle from the autolysosome in the absence of the retromer function, leading to impaired biogenesis of autophagosomes. We therefore conclude that retromer is essential for autophagy-dependent plant infection by the rice blast fungus. © 2015 Zheng et al.
dc.publisherPublic Library of Science
dc.sourceUnpaywall 20200320
dc.subjectfat droplet
dc.subjectglycogen
dc.subjectamino acid sequence
dc.subjectautophagy
dc.subjectcell vacuole
dc.subjectgenetics
dc.subjecthost pathogen interaction
dc.subjectMagnaporthe
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectOryza
dc.subjectpathogenicity
dc.subjectplant disease
dc.subjectplant leaf
dc.subjectprotein transport
dc.subjecttrans Golgi network
dc.subjectAmino Acid Sequence
dc.subjectAutophagy
dc.subjectGlycogen
dc.subjectHost-Pathogen Interactions
dc.subjectLipid Droplets
dc.subjectMagnaporthe
dc.subjectOryza
dc.subjectPlant Diseases
dc.subjectPlant Leaves
dc.subjectProtein Transport
dc.subjecttrans-Golgi Network
dc.subjectVacuoles
dc.typeArticle
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.1371/journal.pgen.1005704
dc.description.sourcetitlePLoS Genetics
dc.description.volume11
dc.description.issue12
dc.description.pagee1005704
dc.published.statePublished
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