Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1006195
Title: Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit
Authors: CHIN CHEEN FEI 
Tan K.
Onishi M.
Chew Y.
Augustine B.
Lee W.R.
Yeong F.M. 
Keywords: chitin synthase
Chs2p protein
Chs3p protein
Fks1p protein
myosin adenosine triphosphatase
unclassified drug
chitin
chitin synthase
Chs2 protein, S cerevisiae
CHS3 protein, S cerevisiae
echinocandin
FKS1 protein, S cerevisiae
glucosyltransferase
membrane protein
myosin subfragment
Saccharomyces cerevisiae protein
Article
cytokinesis
endocytosis
enzyme inhibition
gene deletion
mitosis
mitosis spindle
mitotic spindle premature disassembly
nonhuman
protein secretion
Saccharomyces cerevisiae
sister chromatid
cell cycle
cell membrane
endocytosis
fluorescence microscopy
genetics
mitosis
spindle apparatus
Cell Cycle
Cell Membrane
Chitin
Chitin Synthase
Cytokinesis
Echinocandins
Endocytosis
Glucosyltransferases
Membrane Proteins
Microscopy, Fluorescence
Mitosis
Myosin Subfragments
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Spindle Apparatus
Issue Date: 2016
Publisher: Public Library of Science
Citation: CHIN CHEEN FEI, Tan K., Onishi M., Chew Y., Augustine B., Lee W.R., Yeong F.M. (2016). Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit. PLoS Genetics 12 (7) : e1006195. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1006195
Abstract: Cytokinesis requires the spatio-temporal coordination of membrane deposition and primary septum (PS) formation at the division site to drive acto-myosin ring (AMR) constriction. It has been demonstrated that AMR constriction invariably occurs only after the mitotic spindle disassembly. It has also been established that Chitin Synthase II (Chs2p) neck localization precedes mitotic spindle disassembly during mitotic exit. As AMR constriction depends upon PS formation, the question arises as to how chitin deposition is regulated so as to prevent premature AMR constriction and mitotic spindle breakage. In this study, we propose that cells regulate the coordination between spindle disassembly and AMR constriction via timely endocytosis of cytokinetic enzymes, Chs2p, Chs3p, and Fks1p. Inhibition of endocytosis leads to over accumulation of cytokinetic enzymes during mitotic exit, which accelerates the constriction of the AMR, and causes spindle breakage that eventually could contribute to monopolar spindle formation in the subsequent round of cell division. Intriguingly, the mitotic spindle breakage observed in endocytosis mutants can be rescued either by deleting or inhibiting the activities of, CHS2, CHS3 and FKS1, which are involved in septum formation. The findings from our study highlight the importance of timely endocytosis of cytokinetic enzymes at the division site in safeguarding mitotic spindle integrity during mitotic exit. © 2016 Chin et al.
Source Title: PLoS Genetics
URI: https://scholarbank.nus.edu.sg/handle/10635/165383
ISSN: 15537390
DOI: 10.1371/journal.pgen.1006195
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