Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pbio.2002176
Title: Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators
Authors: Bai Z. 
Chai X.-R. 
Yoon M.J. 
Kim H.-J. 
Lo K.A.
Zhang Z.-C.
Xu D. 
Siang D.T.C. 
Walet A.C.E. 
Xu S.-H. 
Chia S.-Y.
Chen P.
Yang H.
Ghosh S. 
Sun L. 
Keywords: adipocytokine
beta adrenergic receptor stimulating agent
CELF1 protein
cyclic AMP responsive element binding protein
long untranslated RNA
peroxisome proliferator activated receptor gamma
peroxisome proliferator activated receptor gamma coactivator 1alpha
uncoupling protein 1
CELF1 protein
cyclic AMP
cyclic AMP responsive element binding protein
long untranslated RNA
peroxisome proliferator activated receptor gamma coactivator 1alpha
transcriptome
adipocyte
adipose tissue
animal cell
animal experiment
animal model
animal tissue
Article
binding site
cell differentiation
cell structure
citric acid cycle
cold exposure
controlled study
down regulation
energy expenditure
exercise intensity
fatty acid oxidation
gene expression
glycation
lipid storage
long term exposure
male
mouse
nonhuman
oxidative phosphorylation
promoter region
protein expression
protein RNA binding
respiratory chain
RNA sequence
signal transduction
tissue differentiation
upregulation
white adipose tissue
adipose tissue
animal
C57BL mouse
energy metabolism
gene expression regulation
HEK293 cell line
human
metabolism
primary cell culture
Adipose Tissue
Animals
CELF1 Protein
Cell Differentiation
Cyclic AMP
Cyclic AMP Response Element-Binding Protein
Energy Metabolism
Gene Expression Regulation
HEK293 Cells
Humans
Male
Mice, Inbred C57BL
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Primary Cell Culture
RNA, Long Noncoding
Signal Transduction
Transcriptome
Issue Date: 2017
Publisher: Public Library of Science
Citation: Bai Z., Chai X.-R., Yoon M.J., Kim H.-J., Lo K.A., Zhang Z.-C., Xu D., Siang D.T.C., Walet A.C.E., Xu S.-H., Chia S.-Y., Chen P., Yang H., Ghosh S., Sun L. (2017). Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators. PLoS Biology 15 (8) : e2002176. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pbio.2002176
Abstract: Enhancing brown fat activity and promoting white fat browning are attractive therapeutic strategies for treating obesity and associated metabolic disorders. To provide a comprehensive picture of the gene regulatory network in these processes, we conducted a series of transcriptome studies by RNA sequencing (RNA-seq) and quantified the mRNA and long noncoding RNA (lncRNA) changes during white fat browning (chronic cold exposure, beta-adrenergic agonist treatment, and intense exercise) and brown fat activation or inactivation (acute cold exposure or thermoneutrality, respectively). mRNA–lncRNA coexpression networks revealed dynamically regulated lncRNAs to be largely embedded in nutrient and energy metabolism pathways. We identified a brown adipose tissue–enriched lncRNA, lncBATE10, that was governed by the cAMP-cAMP response element-binding protein (Creb) axis and required for a full brown fat differentiation and white fat browning program. Mechanistically, lncBATE10 can decoy Celf1 from Pgc1α, thereby protecting Pgc1α mRNA from repression by Celf1. Together, these studies provide a comprehensive data framework to interrogate the transcriptomic changes accompanying energy homeostasis transition in adipose tissue. © 2017 Bai et al.
Source Title: PLoS Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/165372
ISSN: 15449173
DOI: 10.1371/journal.pbio.2002176
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