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https://doi.org/10.1371/journal.pbio.2002176
Title: | Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators | Authors: | Bai Z. Chai X.-R. Yoon M.J. Kim H.-J. Lo K.A. Zhang Z.-C. Xu D. Siang D.T.C. Walet A.C.E. Xu S.-H. Chia S.-Y. Chen P. Yang H. Ghosh S. Sun L. |
Keywords: | adipocytokine beta adrenergic receptor stimulating agent CELF1 protein cyclic AMP responsive element binding protein long untranslated RNA peroxisome proliferator activated receptor gamma peroxisome proliferator activated receptor gamma coactivator 1alpha uncoupling protein 1 CELF1 protein cyclic AMP cyclic AMP responsive element binding protein long untranslated RNA peroxisome proliferator activated receptor gamma coactivator 1alpha transcriptome adipocyte adipose tissue animal cell animal experiment animal model animal tissue Article binding site cell differentiation cell structure citric acid cycle cold exposure controlled study down regulation energy expenditure exercise intensity fatty acid oxidation gene expression glycation lipid storage long term exposure male mouse nonhuman oxidative phosphorylation promoter region protein expression protein RNA binding respiratory chain RNA sequence signal transduction tissue differentiation upregulation white adipose tissue adipose tissue animal C57BL mouse energy metabolism gene expression regulation HEK293 cell line human metabolism primary cell culture Adipose Tissue Animals CELF1 Protein Cell Differentiation Cyclic AMP Cyclic AMP Response Element-Binding Protein Energy Metabolism Gene Expression Regulation HEK293 Cells Humans Male Mice, Inbred C57BL Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Primary Cell Culture RNA, Long Noncoding Signal Transduction Transcriptome |
Issue Date: | 2017 | Publisher: | Public Library of Science | Citation: | Bai Z., Chai X.-R., Yoon M.J., Kim H.-J., Lo K.A., Zhang Z.-C., Xu D., Siang D.T.C., Walet A.C.E., Xu S.-H., Chia S.-Y., Chen P., Yang H., Ghosh S., Sun L. (2017). Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators. PLoS Biology 15 (8) : e2002176. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pbio.2002176 | Abstract: | Enhancing brown fat activity and promoting white fat browning are attractive therapeutic strategies for treating obesity and associated metabolic disorders. To provide a comprehensive picture of the gene regulatory network in these processes, we conducted a series of transcriptome studies by RNA sequencing (RNA-seq) and quantified the mRNA and long noncoding RNA (lncRNA) changes during white fat browning (chronic cold exposure, beta-adrenergic agonist treatment, and intense exercise) and brown fat activation or inactivation (acute cold exposure or thermoneutrality, respectively). mRNA–lncRNA coexpression networks revealed dynamically regulated lncRNAs to be largely embedded in nutrient and energy metabolism pathways. We identified a brown adipose tissue–enriched lncRNA, lncBATE10, that was governed by the cAMP-cAMP response element-binding protein (Creb) axis and required for a full brown fat differentiation and white fat browning program. Mechanistically, lncBATE10 can decoy Celf1 from Pgc1α, thereby protecting Pgc1α mRNA from repression by Celf1. Together, these studies provide a comprehensive data framework to interrogate the transcriptomic changes accompanying energy homeostasis transition in adipose tissue. © 2017 Bai et al. | Source Title: | PLoS Biology | URI: | https://scholarbank.nus.edu.sg/handle/10635/165372 | ISSN: | 15449173 | DOI: | 10.1371/journal.pbio.2002176 |
Appears in Collections: | Staff Publications Elements |
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